Potassium and calcium channel gene expression in small arteries in porcine and rat models of diet-induced obesity (Poster)

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Lars Jørn Jensen, Max Salomonsson, Charlotte Mehlin Sørensen, Niels-Henrik von Holstein-Rathlou, Henrik Duelund Pedersen, Berit Ø. Christoffersen, Lisbeth Høier Olsen, Trine Pagh Ludvigsen

Obesity is an increasing problem worldwide leading to cardiovascular morbidity. Only limited information exists on the transcriptional regulation of arterial K+ and Ca2+ channels in obesity. We quantified, by real-time PCR, mRNA expression of K+ channels and L-type Ca2+ channels (LTCC) in small mesenteric (MA), middle cerebral (MCA), and left coronary arteries (LCA) of lean vs. obese rats and minipigs. Male Sprague Dawley rats were fed a high-fat (FAT; N=5), high-fructose (FRUC; N=7), high-fat/high-fructose (FAT/FRUC; N=7) or standard diet (STD; N=7-11) for 28 Weeks. FAT and FAT/FRUC became obese, whereas FRUC and STD were lean. Systolic blood pressure (SBP) averaged over 14 weeks was increased (P<0.001) in FRUC and FAT/FRUC but not in FAT. Kir, Kv1.2, Kv1.5, and Kv7.4 channels were up-regulated in FAT, whereas SKca and IKca channels were down-regulated in FAT/FRUC (P<0.05). There were no transcriptional changes in FRUC. Castrated male Göttingen minipigs with or without diabetes were fed a diet rich in fat, cholesterol and fructose (OB+DIAB; N=3 vs. OB; N=2, respectively) or a standard diet (STD; N=3) for 22-45 weeks. Body weight, total body fat content and plasma triglyceride levels were increased in OB and OB+DIAB. BKca, IKca, SKca and/or LTCC mRNA was up-regulated in LCA from OB and OB+DIAB (n.s.). Expression of BKca mRNA was increased, whereas IKca mRNA decreased in MCA from OB (n.s.). SKca mRNA was decreased in MA from OB (n.s.). Diet-induced obesity in rats and minipigs lead to complex changes in K+ and Ca2+ channel gene expression, which, in rats, did not seem to be linked with changes in SBP. These transcriptional changes may lead to disturbances in microvascular flow patterns in obesity.
OriginalsprogEngelsk
Artikelnummer676.4
TidsskriftFASEB journal : official publication of the Federation of American Societies for Experimental Biology
Vol/bind28
Udgave nummerSupplement 1
Antal sider1
ISSN0892-6638
DOI
StatusUdgivet - 27 apr. 2014
BegivenhedExperimental Biology - San Diego, USA
Varighed: 26 apr. 201430 apr. 2014

Konference

KonferenceExperimental Biology
LandUSA
BySan Diego
Periode26/04/201430/04/2014

ID: 139851558