Increased mitochondrial substrate sensitivity in skeletal muscle of patients with type 2 diabetes

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S Larsen, N Stride, Martin Hey-Mogensen, C N Hansen, J L Andersen, S Madsbad, D Worm, J W Helge, F Dela

Mitochondrial respiration has been linked to insulin resistance. We studied mitochondrial respiratory capacity and substrate sensitivity in patients with type 2 diabetes (patients), and obese and lean control participants.
Mitochondrial respiration was measured in permeabilised muscle fibres by respirometry. Protocols for respirometry included titration of substrates for complex I (glutamate), complex II (succinate) and both (octanoyl-carnitine). Myosin heavy chain (MHC) composition, antioxidant capacity (manganese superoxide dismutase [MnSOD]), citrate synthase activity and maximal oxygen uptake (VO2) were also determined. Insulin sensitivity was determined with the isoglycaemic-hyperinsulinaemic clamp technique.
Insulin sensitivity was different (p < 0.05) between the groups (patientsCONCLUSIONS/INTERPRETATION:
Increased mitochondrial substrate sensitivity is seen in skeletal muscle from type 2 diabetic patients and is confined to non-lipid substrates. Respiratory capacity per mitochondrion is not decreased with insulin resistance.
Udgave nummer6
Sider (fra-til)1427-36
Antal sider10
StatusUdgivet - 2011

ID: 33837560