Synthesis of biologically active porcine secretin and [ITyr10] porcine secretin
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Porcine secretin, [Tyr10] secretin, and [Tyr13] secretin were synthesized by solid phase methodology and purified by stepwise gradient elution from a short reversed-phase column with ethanol and acetic acid as organic modifiers. [Tyr10] secretin and [Tyr13] secretin were iodinated by the chloramine-T method and nonmono-, and di-iodinated products separated and isolated by reversed-phase HPLC. Batch incubation analysis is isolated mouse pancreatic islets revealed that secretin and the [Tyr10] analogue were indistinguishable in their effect on the glucose-induced insulin release and cAMP accumulation. [Tyr13] secretin in contrast was significantly less potent in its effect on the glucose-induced insulin release.
Originalsprog | Engelsk |
---|---|
Tidsskrift | International Journal of Peptide and Protein Research |
Vol/bind | 37 |
Udgave nummer | 3 |
Sider (fra-til) | 185-90 |
Antal sider | 6 |
Status | Udgivet - mar. 1991 |
ID: 45574735