Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?
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Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses? / Stavnsbjerg, Camilla; Jørgensen, Jennifer Solgaard; Engel, Trine Bjørnbo; Brus, Anja; Ringgaard, Lars; Hansen, Anders Elias; Kjaer, Andreas; Andresen, Thomas Lars.
In: Journal of Immunological Methods, Vol. 500, 113177, 2022, p. 1-4.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?
AU - Stavnsbjerg, Camilla
AU - Jørgensen, Jennifer Solgaard
AU - Engel, Trine Bjørnbo
AU - Brus, Anja
AU - Ringgaard, Lars
AU - Hansen, Anders Elias
AU - Kjaer, Andreas
AU - Andresen, Thomas Lars
PY - 2022
Y1 - 2022
N2 - Quantification of cytokines in cancerous tissue is important for understanding basic tumor biology and for deciphering anti-cancer mechanisms in drug development. Cytokine measurements on protein-level are often done by immunoassays such as enzyme-linked immunosorbent assay (ELISAs) and multiplex assays. However, immunoassays are prone to interference due to the presence of perturbing factors. The sum of these factors is known as the matrix effect, which results in a deviation of the measured cytokine concentration from the actual concentration. In this study, we demonstrated that matrix effects are present in tumor lysates from 11 different syngeneic murine tumors and that it can greatly affect cytokine measurements in ELISAs and multiplex assays. Dilution of tumor lysates and careful selection of lysis buffer components may decrease matrix effects. However, matrix effects are still present, and care should be taken when analyzing cytokine measurements of tumor lysates.
AB - Quantification of cytokines in cancerous tissue is important for understanding basic tumor biology and for deciphering anti-cancer mechanisms in drug development. Cytokine measurements on protein-level are often done by immunoassays such as enzyme-linked immunosorbent assay (ELISAs) and multiplex assays. However, immunoassays are prone to interference due to the presence of perturbing factors. The sum of these factors is known as the matrix effect, which results in a deviation of the measured cytokine concentration from the actual concentration. In this study, we demonstrated that matrix effects are present in tumor lysates from 11 different syngeneic murine tumors and that it can greatly affect cytokine measurements in ELISAs and multiplex assays. Dilution of tumor lysates and careful selection of lysis buffer components may decrease matrix effects. However, matrix effects are still present, and care should be taken when analyzing cytokine measurements of tumor lysates.
U2 - 10.1016/j.jim.2021.113177
DO - 10.1016/j.jim.2021.113177
M3 - Journal article
C2 - 34756881
VL - 500
SP - 1
EP - 4
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
SN - 0022-1759
M1 - 113177
ER -
ID: 283130190