TY - JOUR

T1 - Viral GPCR US28 can signal in response to chemokine agonists of nearly unlimited structural degeneracy

AU - Miles, Timothy F.

AU - Spiess, Katja

AU - Jude, Kevin M.

AU - Tsutsumi, Naotaka

AU - Burg, John S.

AU - Ingram, Jessica R.

AU - Waghray, Deepa

AU - Hjorto, Gertrud M.

AU - Larsen, Olav

AU - Ploegh, Hidde L.

AU - Rosenkilde, Mette M.

AU - Garcia, K. Christopher

PY - 2018

Y1 - 2018

N2 - Human cytomegalovirus has hijacked and evolved a human G-protein-coupled receptor into US28, which functions as a promiscuous chemokine 'sink' to facilitate evasion of host immune responses. To probe the molecular basis of US28's unique ligand cross-reactivity, we deep sequenced CX3CL1 chemokine libraries selected on 'molecular casts' of the US28 active-state and find that US28 can engage thousands of distinct chemokine sequences, many of which elicit diverse signaling outcomes. The structure of a G-protein-biased CX3CL1-variant in complex with US28 revealed an entirely unique chemokine amino terminal peptide conformation and remodeled constellation of receptor-ligand interactions. Receptor signaling, however, is remarkably robust to mutational disruption of these interactions. Thus, US28 accommodates and functionally discriminates amongst highly degenerate chemokine sequences by sensing the steric bulk of the ligands, which distort both receptor extracellular loops and the walls of the ligand binding pocket to varying degrees, rather than requiring sequence-specific bonding chemistries for recognition and signaling

AB - Human cytomegalovirus has hijacked and evolved a human G-protein-coupled receptor into US28, which functions as a promiscuous chemokine 'sink' to facilitate evasion of host immune responses. To probe the molecular basis of US28's unique ligand cross-reactivity, we deep sequenced CX3CL1 chemokine libraries selected on 'molecular casts' of the US28 active-state and find that US28 can engage thousands of distinct chemokine sequences, many of which elicit diverse signaling outcomes. The structure of a G-protein-biased CX3CL1-variant in complex with US28 revealed an entirely unique chemokine amino terminal peptide conformation and remodeled constellation of receptor-ligand interactions. Receptor signaling, however, is remarkably robust to mutational disruption of these interactions. Thus, US28 accommodates and functionally discriminates amongst highly degenerate chemokine sequences by sensing the steric bulk of the ligands, which distort both receptor extracellular loops and the walls of the ligand binding pocket to varying degrees, rather than requiring sequence-specific bonding chemistries for recognition and signaling

U2 - 10.7554/eLife.35850

DO - 10.7554/eLife.35850

M3 - Journal article

C2 - 29882741

VL - 7

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e35850

ER -