Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity

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Standard

Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity. / Mandrup-Poulsen, T; Egeberg, J; Nerup, J; Bendtzen, K; Dinarello, C A; Nielsen, Jens Høiriis.

I: Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, Bind 95, Nr. 2, 04.1987, s. 55-63.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Mandrup-Poulsen, T, Egeberg, J, Nerup, J, Bendtzen, K, Dinarello, CA & Nielsen, JH 1987, 'Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity', Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, bind 95, nr. 2, s. 55-63.

APA

Mandrup-Poulsen, T., Egeberg, J., Nerup, J., Bendtzen, K., Dinarello, C. A., & Nielsen, J. H. (1987). Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity. Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology, 95(2), 55-63.

Vancouver

Mandrup-Poulsen T, Egeberg J, Nerup J, Bendtzen K, Dinarello CA, Nielsen JH. Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity. Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 1987 apr;95(2):55-63.

Author

Mandrup-Poulsen, T ; Egeberg, J ; Nerup, J ; Bendtzen, K ; Dinarello, C A ; Nielsen, Jens Høiriis. / Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity. I: Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology. 1987 ; Bind 95, Nr. 2. s. 55-63.

Bibtex

@article{870349a634494e7faba78385c4ff528c,
title = "Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity",
abstract = "Previous electron-microscopic studies of isolated islets of Langerhans exposed to the monokine interleukin-1 for 7 days have indicated that interleukin-1 is cytotoxic to all islet cells. To study the time-course and possible cellular specificity of interleukin-1 cytotoxicity to islets exposed to interleukin-1 for short time periods, isolated rat or human islets were incubated with or without 25 U/ml highly purified human interleukin-1 for 24 h. Samples of rat islets were taken after 5 min, 30 min, 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h and samples of human islets after 5 min, 30 min and 24 h of incubation and examined by electron microscopy in a blinded fashion. Already after 30 min, accumulation of opaque intracytoplasmic bodies without apparent surrounding membranes, and autophagic vacuoles were seen in about 20{\%} of the beta cells examined in rat islets exposed to interleukin-1. After 16 h of incubation with interleukin-1, more than 80{\%} of rat beta cells showed signs of degeneration. Beta cell specific changes similar to those observed in rat islets exposed to IL-1 for 30 min were seen in human islets exposed to IL-1 for 24 h. The described changes were not observed in alpha cells in interleukin-1-treated rat or human islets, or in alpha and beta cells in control islets. Passing interleukin-1 over columns containing Sepharose-coupled anti-interleukin-1 antibody completely removed the beta cell cytotoxic action on rat islets.(ABSTRACT TRUNCATED AT 250 WORDS)",
keywords = "Animals, Cytoplasmic Granules, Cytotoxins, Diabetes Mellitus, Type 1, Humans, Interleukin-1, Islets of Langerhans, Kinetics, Macrophages, Microscopy, Electron, Rats, Vacuoles",
author = "T Mandrup-Poulsen and J Egeberg and J Nerup and K Bendtzen and Dinarello, {C A} and Nielsen, {Jens H{\o}iriis}",
year = "1987",
month = "4",
language = "English",
volume = "95",
pages = "55--63",
journal = "Acta Pathologica Microbiologica et Immunologica Scandinavica - Section C Immunology",
issn = "0108-0202",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Ultrastructural studies of time-course and cellular specificity of interleukin-1 mediated islet cytotoxicity

AU - Mandrup-Poulsen, T

AU - Egeberg, J

AU - Nerup, J

AU - Bendtzen, K

AU - Dinarello, C A

AU - Nielsen, Jens Høiriis

PY - 1987/4

Y1 - 1987/4

N2 - Previous electron-microscopic studies of isolated islets of Langerhans exposed to the monokine interleukin-1 for 7 days have indicated that interleukin-1 is cytotoxic to all islet cells. To study the time-course and possible cellular specificity of interleukin-1 cytotoxicity to islets exposed to interleukin-1 for short time periods, isolated rat or human islets were incubated with or without 25 U/ml highly purified human interleukin-1 for 24 h. Samples of rat islets were taken after 5 min, 30 min, 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h and samples of human islets after 5 min, 30 min and 24 h of incubation and examined by electron microscopy in a blinded fashion. Already after 30 min, accumulation of opaque intracytoplasmic bodies without apparent surrounding membranes, and autophagic vacuoles were seen in about 20% of the beta cells examined in rat islets exposed to interleukin-1. After 16 h of incubation with interleukin-1, more than 80% of rat beta cells showed signs of degeneration. Beta cell specific changes similar to those observed in rat islets exposed to IL-1 for 30 min were seen in human islets exposed to IL-1 for 24 h. The described changes were not observed in alpha cells in interleukin-1-treated rat or human islets, or in alpha and beta cells in control islets. Passing interleukin-1 over columns containing Sepharose-coupled anti-interleukin-1 antibody completely removed the beta cell cytotoxic action on rat islets.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - Previous electron-microscopic studies of isolated islets of Langerhans exposed to the monokine interleukin-1 for 7 days have indicated that interleukin-1 is cytotoxic to all islet cells. To study the time-course and possible cellular specificity of interleukin-1 cytotoxicity to islets exposed to interleukin-1 for short time periods, isolated rat or human islets were incubated with or without 25 U/ml highly purified human interleukin-1 for 24 h. Samples of rat islets were taken after 5 min, 30 min, 1, 2, 4, 6, 8, 10, 12, 16, 20 and 24 h and samples of human islets after 5 min, 30 min and 24 h of incubation and examined by electron microscopy in a blinded fashion. Already after 30 min, accumulation of opaque intracytoplasmic bodies without apparent surrounding membranes, and autophagic vacuoles were seen in about 20% of the beta cells examined in rat islets exposed to interleukin-1. After 16 h of incubation with interleukin-1, more than 80% of rat beta cells showed signs of degeneration. Beta cell specific changes similar to those observed in rat islets exposed to IL-1 for 30 min were seen in human islets exposed to IL-1 for 24 h. The described changes were not observed in alpha cells in interleukin-1-treated rat or human islets, or in alpha and beta cells in control islets. Passing interleukin-1 over columns containing Sepharose-coupled anti-interleukin-1 antibody completely removed the beta cell cytotoxic action on rat islets.(ABSTRACT TRUNCATED AT 250 WORDS)

KW - Animals

KW - Cytoplasmic Granules

KW - Cytotoxins

KW - Diabetes Mellitus, Type 1

KW - Humans

KW - Interleukin-1

KW - Islets of Langerhans

KW - Kinetics

KW - Macrophages

KW - Microscopy, Electron

KW - Rats

KW - Vacuoles

M3 - Journal article

C2 - 3300162

VL - 95

SP - 55

EP - 63

JO - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section C Immunology

JF - Acta Pathologica Microbiologica et Immunologica Scandinavica - Section C Immunology

SN - 0108-0202

IS - 2

ER -

ID: 47974927