Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus

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Standard

Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus. / Kristensen, P; Larsson, L I; Danø, K; Nielsen, Jens Høiriis.

I: Endocrinology, Bind 121, Nr. 6, 12.1987, s. 2238-44.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Kristensen, P, Larsson, LI, Danø, K & Nielsen, JH 1987, 'Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus', Endocrinology, bind 121, nr. 6, s. 2238-44.

APA

Kristensen, P., Larsson, L. I., Danø, K., & Nielsen, J. H. (1987). Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus. Endocrinology, 121(6), 2238-44.

Vancouver

Kristensen P, Larsson LI, Danø K, Nielsen JH. Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus. Endocrinology. 1987 dec;121(6):2238-44.

Author

Kristensen, P ; Larsson, L I ; Danø, K ; Nielsen, Jens Høiriis. / Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus. I: Endocrinology. 1987 ; Bind 121, Nr. 6. s. 2238-44.

Bibtex

@article{868132e1aa324501ade624c49695f774,
title = "Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus",
abstract = "Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.",
keywords = "Animals, Hypothalamus, Immunoenzyme Techniques, Islets of Langerhans, Male, Median Eminence, Molecular Weight, Rats, Rats, Inbred Strains, Somatostatin, Tissue Plasminogen Activator",
author = "P Kristensen and Larsson, {L I} and K Dan{\o} and Nielsen, {Jens H{\o}iriis}",
year = "1987",
month = "12",
language = "English",
volume = "121",
pages = "2238--44",
journal = "Endocrinology",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Tissue-type plasminogen activator in somatostatin cells of rat pancreas and hypothalamus

AU - Kristensen, P

AU - Larsson, L I

AU - Danø, K

AU - Nielsen, Jens Høiriis

PY - 1987/12

Y1 - 1987/12

N2 - Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.

AB - Plasminogen activators (PAs) proteolytically convert plasminogen to plasmin, which, in turn, can degrade most proteins. This system has been implicated in a variety of biological processes. Using immunocytochemical methods, we here describe the localization of tissue-type PA (t-PA) in rat somatostatin cells. In the pancreatic islets, a low number of strongly t-PA-immunoreactive cells was found. By sequential staining, we found these cells to constitute a subpopulation of the somatostatin cells. Biochemical analysis of extracts from isolated rat islets demonstrated the presence of t-PA, and immunoblotting analysis demonstrated one band with a similar electrophoretic mobility. No urokinase-type PA immunoreactivity was found in the rat endocrine pancreas. A granular t-PA immunoreactivity resembling that found in adjacent sections with somatostatin antiserum was found in the median eminence of the hypothalamus. No t-PA immunoreactivity could be detected in somatostatin cells of the gastric and intestinal mucosa.

KW - Animals

KW - Hypothalamus

KW - Immunoenzyme Techniques

KW - Islets of Langerhans

KW - Male

KW - Median Eminence

KW - Molecular Weight

KW - Rats

KW - Rats, Inbred Strains

KW - Somatostatin

KW - Tissue Plasminogen Activator

M3 - Journal article

VL - 121

SP - 2238

EP - 2244

JO - Endocrinology

JF - Endocrinology

SN - 0013-7227

IS - 6

ER -

ID: 47974815