Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites

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Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites. / Trammell, Samuel A.J.; Brenner, Charles.

I: Computational and Structural Biotechnology Journal, Bind 4, Nr. 5, e201301012, 2013.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Trammell, SAJ & Brenner, C 2013, 'Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites', Computational and Structural Biotechnology Journal, bind 4, nr. 5, e201301012. https://doi.org/10.5936/csbj.201301012

APA

Trammell, S. A. J., & Brenner, C. (2013). Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites. Computational and Structural Biotechnology Journal, 4(5), [e201301012]. https://doi.org/10.5936/csbj.201301012

Vancouver

Trammell SAJ, Brenner C. Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites. Computational and Structural Biotechnology Journal. 2013;4(5). e201301012. https://doi.org/10.5936/csbj.201301012

Author

Trammell, Samuel A.J. ; Brenner, Charles. / Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites. I: Computational and Structural Biotechnology Journal. 2013 ; Bind 4, Nr. 5.

Bibtex

@article{523c1647007c47729a57ac5a28d029d0,
title = "Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites",
abstract = "Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.",
author = "Trammell, {Samuel A.J.} and Charles Brenner",
year = "2013",
doi = "10.5936/csbj.201301012",
language = "English",
volume = "4",
journal = "Computational and Structural Biotechnology Journal",
issn = "2001-0370",
publisher = "Research Network of Computational and Structural Biotechnology (RNCSB)",
number = "5",

}

RIS

TY - JOUR

T1 - Targeted, LCMS-based metabolomics for quantitative measurement of NAD+ metabolites

AU - Trammell, Samuel A.J.

AU - Brenner, Charles

PY - 2013

Y1 - 2013

N2 - Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.

AB - Nicotinamide adenine dinucleotide (NAD+) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD+-consuming enzymes. The abundance of NAD +, NAD+ biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysis of NAD+ metabolites does not offer the specificity and sensitivity necessary for robust quantification of complex samples. Thus, we developed a targeted, quantitative assay of the NAD+ metabolome with the use of HPLC coupled to mass spectrometry. Here we discuss NAD+ metabolism as well as the technical challenges required for reliable quantification of the NAD+ metabolites. The new method incorporates new separations and improves upon a previously published method that suffered from the problem of ionization suppression for particular compounds.

U2 - 10.5936/csbj.201301012

DO - 10.5936/csbj.201301012

M3 - Journal article

AN - SCOPUS:84888626285

VL - 4

JO - Computational and Structural Biotechnology Journal

JF - Computational and Structural Biotechnology Journal

SN - 2001-0370

IS - 5

M1 - e201301012

ER -

ID: 220855687