Respiratory syncytial virus infection facilitates acute colonization of Pseudomonas aeruginosa in mice
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Respiratory syncytial virus infection facilitates acute colonization of Pseudomonas aeruginosa in mice. / de Vrankrijker, Angélica M M; Wolfs, Tom F W; Ciofu, Oana; Høiby, Niels; van der Ent, Cornelis K; Poulsen, Steen S; Johansen, Helle Krogh.
I: Journal of Medical Virology, Bind 81, Nr. 12, 2009, s. 2096-103.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Respiratory syncytial virus infection facilitates acute colonization of Pseudomonas aeruginosa in mice
AU - de Vrankrijker, Angélica M M
AU - Wolfs, Tom F W
AU - Ciofu, Oana
AU - Høiby, Niels
AU - van der Ent, Cornelis K
AU - Poulsen, Steen S
AU - Johansen, Helle Krogh
N1 - Keywords: Acute Disease; Animals; Bronchoalveolar Lavage Fluid; Cytokines; Female; Humans; Lung; Mice; Mice, Inbred BALB C; Pseudomonas Infections; Pseudomonas aeruginosa; Respiratory Syncytial Virus Infections; Respiratory Syncytial Viruses; Virulence
PY - 2009
Y1 - 2009
N2 - Pseudomonas aeruginosa causes opportunistic infections in immunocompromised individuals and patients ventilated mechanically and is the major pathogen in patients with cystic fibrosis, in which it causes chronic infections. Epidemiological, in vitro and animal data suggest a role for respiratory virus infections in facilitating colonization and infection with P. aeruginosa. A study was undertaken to determine whether respiratory syncytial virus (RSV) infection could facilitate the initiation of an acute infection with P. aeruginosa in vivo. Balb/c mice were infected intranasally with P. aeruginosa, with and without simultaneous inoculation with RSV. Lung function measurements were undertaken using Whole Body Plethysmography and lungs were harvested 24 hr after inoculation. Mice exposed to RSV and P. aeruginosa showed 2,000 times higher colony-forming units (CFU) counts of P. aeruginosa in the lung homogenates when compared to mice which were only infected with P. aeruginosa and lung function changes were more severe in co-infected mice. Control mice receiving RSV alone showed no significant changes in lung function or cytokine production, and no inflammatory changes in the lung parenchyma. These results suggest that RSV can facilitate the initiation of acute P. aeruginosa infection without the RSV infection being clinically apparent. This could have implications for treatment strategies to prevent opportunistic P. aeruginosa lung infection.
AB - Pseudomonas aeruginosa causes opportunistic infections in immunocompromised individuals and patients ventilated mechanically and is the major pathogen in patients with cystic fibrosis, in which it causes chronic infections. Epidemiological, in vitro and animal data suggest a role for respiratory virus infections in facilitating colonization and infection with P. aeruginosa. A study was undertaken to determine whether respiratory syncytial virus (RSV) infection could facilitate the initiation of an acute infection with P. aeruginosa in vivo. Balb/c mice were infected intranasally with P. aeruginosa, with and without simultaneous inoculation with RSV. Lung function measurements were undertaken using Whole Body Plethysmography and lungs were harvested 24 hr after inoculation. Mice exposed to RSV and P. aeruginosa showed 2,000 times higher colony-forming units (CFU) counts of P. aeruginosa in the lung homogenates when compared to mice which were only infected with P. aeruginosa and lung function changes were more severe in co-infected mice. Control mice receiving RSV alone showed no significant changes in lung function or cytokine production, and no inflammatory changes in the lung parenchyma. These results suggest that RSV can facilitate the initiation of acute P. aeruginosa infection without the RSV infection being clinically apparent. This could have implications for treatment strategies to prevent opportunistic P. aeruginosa lung infection.
U2 - 10.1002/jmv.21623
DO - 10.1002/jmv.21623
M3 - Journal article
C2 - 19856469
VL - 81
SP - 2096
EP - 2103
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 12
ER -
ID: 18787061