Renal origin of rat urinary epidermal growth factor

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Standard

Renal origin of rat urinary epidermal growth factor. / Nexø, Ebba; Poulsen, Steen Seier.

I: Regulatory Peptides, Bind 10, Nr. 1, 12.1984, s. 37-45.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Nexø, E & Poulsen, SS 1984, 'Renal origin of rat urinary epidermal growth factor', Regulatory Peptides, bind 10, nr. 1, s. 37-45.

APA

Nexø, E., & Poulsen, S. S. (1984). Renal origin of rat urinary epidermal growth factor. Regulatory Peptides, 10(1), 37-45.

Vancouver

Nexø E, Poulsen SS. Renal origin of rat urinary epidermal growth factor. Regulatory Peptides. 1984 dec.;10(1):37-45.

Author

Nexø, Ebba ; Poulsen, Steen Seier. / Renal origin of rat urinary epidermal growth factor. I: Regulatory Peptides. 1984 ; Bind 10, Nr. 1. s. 37-45.

Bibtex

@article{ca8c2ed77f124882bbdbff23f9d9edfa,
title = "Renal origin of rat urinary epidermal growth factor",
abstract = "The origin of rat urinary epidermal growth factor (EGF) has been investigated. Unilateral nephrectomy decreased the concentration, total output of EGF and EGF/creatinine ratio by approximately 50%, while the output of creatinine was unchanged. Removal of the submandibular glands and duodenal Brunner's glands, organs known to produce EGF, had no influence on the output of EGF in urine. Renal clearance of EGF exceeded that of creatinine, and after bilateral nephrectomy or bilateral ligation of the ureters, the concentration of creatinine in serum increased, while the concentration of EGF was below the detection limit of the assay. Renal production of EGF was confirmed by immunohistochemistry demonstrating EGF immunoreactivity in the afferent arteriole of the juxtaglomerular apparatus. EGF in the submandibular glands and in urine was found to differ with chromatofocusing and reverse-phase HPLC. At isoelectric focusing the pI of submandibular EGF was 4.8 and 5.4 while that of urinary EGF was 5.3 and 6.4. In conclusion, this study demonstrates that urinary EGF mainly originates from the kidneys and is localized to the renal juxtaglomerular apparatus.",
keywords = "Animals, Chromatography, High Pressure Liquid, Creatinine, Duodenum, Epidermal Growth Factor, Histocytochemistry, Immunochemistry, Isoelectric Focusing, Kidney, Male, Nephrectomy, Rats, Rats, Inbred Strains, Submandibular Gland",
author = "Ebba Nex{\o} and Poulsen, {Steen Seier}",
year = "1984",
month = dec,
language = "English",
volume = "10",
pages = "37--45",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Renal origin of rat urinary epidermal growth factor

AU - Nexø, Ebba

AU - Poulsen, Steen Seier

PY - 1984/12

Y1 - 1984/12

N2 - The origin of rat urinary epidermal growth factor (EGF) has been investigated. Unilateral nephrectomy decreased the concentration, total output of EGF and EGF/creatinine ratio by approximately 50%, while the output of creatinine was unchanged. Removal of the submandibular glands and duodenal Brunner's glands, organs known to produce EGF, had no influence on the output of EGF in urine. Renal clearance of EGF exceeded that of creatinine, and after bilateral nephrectomy or bilateral ligation of the ureters, the concentration of creatinine in serum increased, while the concentration of EGF was below the detection limit of the assay. Renal production of EGF was confirmed by immunohistochemistry demonstrating EGF immunoreactivity in the afferent arteriole of the juxtaglomerular apparatus. EGF in the submandibular glands and in urine was found to differ with chromatofocusing and reverse-phase HPLC. At isoelectric focusing the pI of submandibular EGF was 4.8 and 5.4 while that of urinary EGF was 5.3 and 6.4. In conclusion, this study demonstrates that urinary EGF mainly originates from the kidneys and is localized to the renal juxtaglomerular apparatus.

AB - The origin of rat urinary epidermal growth factor (EGF) has been investigated. Unilateral nephrectomy decreased the concentration, total output of EGF and EGF/creatinine ratio by approximately 50%, while the output of creatinine was unchanged. Removal of the submandibular glands and duodenal Brunner's glands, organs known to produce EGF, had no influence on the output of EGF in urine. Renal clearance of EGF exceeded that of creatinine, and after bilateral nephrectomy or bilateral ligation of the ureters, the concentration of creatinine in serum increased, while the concentration of EGF was below the detection limit of the assay. Renal production of EGF was confirmed by immunohistochemistry demonstrating EGF immunoreactivity in the afferent arteriole of the juxtaglomerular apparatus. EGF in the submandibular glands and in urine was found to differ with chromatofocusing and reverse-phase HPLC. At isoelectric focusing the pI of submandibular EGF was 4.8 and 5.4 while that of urinary EGF was 5.3 and 6.4. In conclusion, this study demonstrates that urinary EGF mainly originates from the kidneys and is localized to the renal juxtaglomerular apparatus.

KW - Animals

KW - Chromatography, High Pressure Liquid

KW - Creatinine

KW - Duodenum

KW - Epidermal Growth Factor

KW - Histocytochemistry

KW - Immunochemistry

KW - Isoelectric Focusing

KW - Kidney

KW - Male

KW - Nephrectomy

KW - Rats

KW - Rats, Inbred Strains

KW - Submandibular Gland

M3 - Journal article

C2 - 6335757

VL - 10

SP - 37

EP - 45

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 1

ER -

ID: 47489179