TY - JOUR

T1 - Planar SERS nanostructures with stochastic silver ring morphology for biosensor chips

AU - Semenova, Anna

AU - Goodilin, Eugene

AU - Brazhe, Nadezda

AU - Ivanov, Vladimir

AU - Baranchikov, Alexander

AU - Lebedev, Vasiliy

AU - Goldt, Anastasia

AU - Sosnovtseva, Olga

AU - Savilov, Sergey

AU - Egorov, Alexander

AU - Brazhe, Alexey

AU - Parshina, Evgeniya

AU - Luneva, Oxana

AU - Maksimov, Georgy

AU - Tretyakov, Yury

PY - 2012

Y1 - 2012

N2 - Surface-enhanced Raman spectroscopy (SERS) of living cells has rapidly become a powerful trend in biomedical diagnostics. It is a common belief that highly ordered, artificially engineered substrates are the best future decision in this field. This paper, however, describes an alternative successful solution, a new effortless chemical approach to the design of nanostructured silver and heterometallic continuous coatings with a stochastic ‘‘coffee ring’’ morphology. The coatings are formed from an ultrasonic mist of aqueous diamminesilver hydroxide, free of reducing agents and nonvolatile pollutants, under mild conditions, at about 200–270 C in air. They consist of 30–100 micrometer wide and 100–400 nm high silver rings composed, in turn, of a porous silver matrix with 10–50 nm silver grains decorating the sponge. This hierarchic structure originates from ultrasonic droplet evaporation, contact-line motion, silver(I) oxide decomposition and evolution of a growing ensemble of silver rings. The fabricated substrates are a remarkable example of a new scalable and low cost material suitable for SERS analysesof living cells. They evoke no hemolysis and reduce erythrocyte lateral mobility due to suitable ‘‘coffee ring’’ sizes and a tight contact with the silver nanostructure. A high SERS enhancement, characteristicof pure silver rings, made it possible to record Raman scattering spectra from submembrane hemoglobin in its natural cellular environment inside single living erythrocytes, thus making the substrates promising for various biosensor chips.Introduction

AB - Surface-enhanced Raman spectroscopy (SERS) of living cells has rapidly become a powerful trend in biomedical diagnostics. It is a common belief that highly ordered, artificially engineered substrates are the best future decision in this field. This paper, however, describes an alternative successful solution, a new effortless chemical approach to the design of nanostructured silver and heterometallic continuous coatings with a stochastic ‘‘coffee ring’’ morphology. The coatings are formed from an ultrasonic mist of aqueous diamminesilver hydroxide, free of reducing agents and nonvolatile pollutants, under mild conditions, at about 200–270 C in air. They consist of 30–100 micrometer wide and 100–400 nm high silver rings composed, in turn, of a porous silver matrix with 10–50 nm silver grains decorating the sponge. This hierarchic structure originates from ultrasonic droplet evaporation, contact-line motion, silver(I) oxide decomposition and evolution of a growing ensemble of silver rings. The fabricated substrates are a remarkable example of a new scalable and low cost material suitable for SERS analysesof living cells. They evoke no hemolysis and reduce erythrocyte lateral mobility due to suitable ‘‘coffee ring’’ sizes and a tight contact with the silver nanostructure. A high SERS enhancement, characteristicof pure silver rings, made it possible to record Raman scattering spectra from submembrane hemoglobin in its natural cellular environment inside single living erythrocytes, thus making the substrates promising for various biosensor chips.Introduction

U2 - 10.1039/c2jm34686a

DO - 10.1039/c2jm34686a

M3 - Journal article

VL - 22

SP - 24530

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 47

ER -