PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise: effect of intralipid infusion
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PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise : effect of intralipid infusion. / Pilegaard, Henriette; Birk, Jesper Bratz; Sacchetti, Massimo; Mourtzakis, Marina; Hardie, D. Graham; Stewart, Greg; Neufer, P. Darrell; Saltin, Bengt; Van Hall, Gerrit; Wojtaszewski, Jørgen.
I: Diabetes, Bind 55, Nr. 11, 2006, s. 3020-3027.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - PDH-E1alpha dephosphorylation and activation in human skeletal muscle during exercise
T2 - effect of intralipid infusion
AU - Pilegaard, Henriette
AU - Birk, Jesper Bratz
AU - Sacchetti, Massimo
AU - Mourtzakis, Marina
AU - Hardie, D. Graham
AU - Stewart, Greg
AU - Neufer, P. Darrell
AU - Saltin, Bengt
AU - Van Hall, Gerrit
AU - Wojtaszewski, Jørgen
N1 - PUF 2006 5200 022
PY - 2006
Y1 - 2006
N2 - To investigate pyruvate dehydrogenase (PDH)-E1a subunit phosphorylation and whether free fatty acids (FFAs) regulate PDH activity, seven subjects completed two trials: saline (control) and intralipid/heparin (intralipid). Each infusion trial consisted of a 4-h rest followed by a 3-h two-legged knee extensor exercise at moderate intensity. During the 4-h resting period, activity of PDH in the active form (PDHa) did not change in either trial, yet phosphorylation of PDH-E1a site 1 (PDH-P1) and site 2 (PDH-P2) was elevated in the intralipid compared with the control trial. PDHa activity increased during exercise similarly in the two trials. After 3 h of exercise, PDHa activity remained elevated in the intralipid trial but returned to resting levels in the control trial. Accordingly, in both trials PDH-P1 and PDH-P2 decreased during exercise, and the decrease was more marked during intralipid infusion. Phosphorylation had returned to resting levels at 3 h of exercise only in the control trial. Thus, an inverse association between PDH-E1a phosphorylation and PDHa activity exists. Short-term elevation in plasma FFA at rest increases PDH-E1a phosphorylation, but exercise overrules this effect of FFA on PDH-E1a phosphorylation leading to even greater dephosphorylation during exercise with intralipid infusion than with saline.
AB - To investigate pyruvate dehydrogenase (PDH)-E1a subunit phosphorylation and whether free fatty acids (FFAs) regulate PDH activity, seven subjects completed two trials: saline (control) and intralipid/heparin (intralipid). Each infusion trial consisted of a 4-h rest followed by a 3-h two-legged knee extensor exercise at moderate intensity. During the 4-h resting period, activity of PDH in the active form (PDHa) did not change in either trial, yet phosphorylation of PDH-E1a site 1 (PDH-P1) and site 2 (PDH-P2) was elevated in the intralipid compared with the control trial. PDHa activity increased during exercise similarly in the two trials. After 3 h of exercise, PDHa activity remained elevated in the intralipid trial but returned to resting levels in the control trial. Accordingly, in both trials PDH-P1 and PDH-P2 decreased during exercise, and the decrease was more marked during intralipid infusion. Phosphorylation had returned to resting levels at 3 h of exercise only in the control trial. Thus, an inverse association between PDH-E1a phosphorylation and PDHa activity exists. Short-term elevation in plasma FFA at rest increases PDH-E1a phosphorylation, but exercise overrules this effect of FFA on PDH-E1a phosphorylation leading to even greater dephosphorylation during exercise with intralipid infusion than with saline.
U2 - 10.2337/db06-0152
DO - 10.2337/db06-0152
M3 - Journal article
VL - 55
SP - 3020
EP - 3027
JO - Diabetes
JF - Diabetes
SN - 0012-1797
IS - 11
ER -
ID: 314331