Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites. / Larsson, L I; Hutton, J C; Madsen, O D; Nielsen, Jens Høiriis.

I: European Journal of Cell Biology, Bind 48, Nr. 1, 02.1989, s. 45-51.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Larsson, LI, Hutton, JC, Madsen, OD & Nielsen, JH 1989, 'Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites', European Journal of Cell Biology, bind 48, nr. 1, s. 45-51.

APA

Larsson, L. I., Hutton, J. C., Madsen, O. D., & Nielsen, J. H. (1989). Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites. European Journal of Cell Biology, 48(1), 45-51.

Vancouver

Larsson LI, Hutton JC, Madsen OD, Nielsen JH. Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites. European Journal of Cell Biology. 1989 feb.;48(1):45-51.

Author

Larsson, L I ; Hutton, J C ; Madsen, O D ; Nielsen, Jens Høiriis. / Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites. I: European Journal of Cell Biology. 1989 ; Bind 48, Nr. 1. s. 45-51.

Bibtex

@article{e1de132ca17b4baea6d643c7a8312832,
title = "Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites",
abstract = "Human and rat insulin cells show insulin immunoreactivity, and glucagon cells show glucagon immunoreactivity on their membrane surfaces, respectively. The reaction occurs in the form of small dots on the islet cell surface and colocalizes with the chromogranin family of secretory granule markers. Electron microscopy reveals the labeling to occur at sites of exocytotic granule release, involving the surfaces of extruded granule cores. The surfaces of islet cells were labeled both by polyclonal and monoclonal antibodies, excluding that receptor-interacting, anti-idiotypic hormone antibodies were responsible for the staining. Human insulin cells were surface-labeled by monoclonal antibodies recognizing the mature secretory products, insulin and C-peptide but not with monoclonal antibodies specific for proinsulin. Thus, routing of unprocessed preproinsulin to the cell surface may not account for these results. It is concluded that the staining reflects interactions between the appropriate antibodies and exocytotic sites of hormone release.",
keywords = "Animals, C-Peptide, Cell Membrane, Chromogranins, Exocytosis, Glucagon, Humans, Immunohistochemistry, Insulin, Islets of Langerhans, Microscopy, Electron, Pancreatic Hormones, Rats, Rats, Inbred Strains",
author = "Larsson, {L I} and Hutton, {J C} and Madsen, {O D} and Nielsen, {Jens H{\o}iriis}",
year = "1989",
month = feb,
language = "English",
volume = "48",
pages = "45--51",
journal = "Cytobiologie",
issn = "0724-5130",
publisher = "Elsevier GmbH - Urban und Fischer",
number = "1",

}

RIS

TY - JOUR

T1 - Pancreatic hormones are expressed on the surfaces of human and rat islet cells through exocytotic sites

AU - Larsson, L I

AU - Hutton, J C

AU - Madsen, O D

AU - Nielsen, Jens Høiriis

PY - 1989/2

Y1 - 1989/2

N2 - Human and rat insulin cells show insulin immunoreactivity, and glucagon cells show glucagon immunoreactivity on their membrane surfaces, respectively. The reaction occurs in the form of small dots on the islet cell surface and colocalizes with the chromogranin family of secretory granule markers. Electron microscopy reveals the labeling to occur at sites of exocytotic granule release, involving the surfaces of extruded granule cores. The surfaces of islet cells were labeled both by polyclonal and monoclonal antibodies, excluding that receptor-interacting, anti-idiotypic hormone antibodies were responsible for the staining. Human insulin cells were surface-labeled by monoclonal antibodies recognizing the mature secretory products, insulin and C-peptide but not with monoclonal antibodies specific for proinsulin. Thus, routing of unprocessed preproinsulin to the cell surface may not account for these results. It is concluded that the staining reflects interactions between the appropriate antibodies and exocytotic sites of hormone release.

AB - Human and rat insulin cells show insulin immunoreactivity, and glucagon cells show glucagon immunoreactivity on their membrane surfaces, respectively. The reaction occurs in the form of small dots on the islet cell surface and colocalizes with the chromogranin family of secretory granule markers. Electron microscopy reveals the labeling to occur at sites of exocytotic granule release, involving the surfaces of extruded granule cores. The surfaces of islet cells were labeled both by polyclonal and monoclonal antibodies, excluding that receptor-interacting, anti-idiotypic hormone antibodies were responsible for the staining. Human insulin cells were surface-labeled by monoclonal antibodies recognizing the mature secretory products, insulin and C-peptide but not with monoclonal antibodies specific for proinsulin. Thus, routing of unprocessed preproinsulin to the cell surface may not account for these results. It is concluded that the staining reflects interactions between the appropriate antibodies and exocytotic sites of hormone release.

KW - Animals

KW - C-Peptide

KW - Cell Membrane

KW - Chromogranins

KW - Exocytosis

KW - Glucagon

KW - Humans

KW - Immunohistochemistry

KW - Insulin

KW - Islets of Langerhans

KW - Microscopy, Electron

KW - Pancreatic Hormones

KW - Rats

KW - Rats, Inbred Strains

M3 - Journal article

C2 - 2663499

VL - 48

SP - 45

EP - 51

JO - Cytobiologie

JF - Cytobiologie

SN - 0724-5130

IS - 1

ER -

ID: 47974343