Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes

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Standard

Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes. / Gorasia, Dhana G; Dudek, Nadine L; Veith, Paul D; Shankar, Renu; Safavi-Hemami, Helena; Williamson, Nicholas A; Reynolds, Eric C; Hubbard, Michael J; Purcell, Anthony W.

I: Journal of Proteome Research, Bind 14, Nr. 2, 2015, s. 688-99.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Gorasia, DG, Dudek, NL, Veith, PD, Shankar, R, Safavi-Hemami, H, Williamson, NA, Reynolds, EC, Hubbard, MJ & Purcell, AW 2015, 'Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes', Journal of Proteome Research, bind 14, nr. 2, s. 688-99. https://doi.org/10.1021/pr500643h

APA

Gorasia, D. G., Dudek, N. L., Veith, P. D., Shankar, R., Safavi-Hemami, H., Williamson, N. A., Reynolds, E. C., Hubbard, M. J., & Purcell, A. W. (2015). Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes. Journal of Proteome Research, 14(2), 688-99. https://doi.org/10.1021/pr500643h

Vancouver

Gorasia DG, Dudek NL, Veith PD, Shankar R, Safavi-Hemami H, Williamson NA o.a. Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes. Journal of Proteome Research. 2015;14(2):688-99. https://doi.org/10.1021/pr500643h

Author

Gorasia, Dhana G ; Dudek, Nadine L ; Veith, Paul D ; Shankar, Renu ; Safavi-Hemami, Helena ; Williamson, Nicholas A ; Reynolds, Eric C ; Hubbard, Michael J ; Purcell, Anthony W. / Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes. I: Journal of Proteome Research. 2015 ; Bind 14, Nr. 2. s. 688-99.

Bibtex

@article{41883cb553a1406896952d0ab09106f1,
title = "Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes",
abstract = "The complex interplay of many cell types and the temporal heterogeneity of pancreatic islet composition obscure the direct role of resident alpha and beta cells in the development of Type 1 diabetes. Therefore, in addition to studying islets isolated from non-obese diabetic mice, we analyzed homogeneous cell populations of murine alpha (αTC-1) and beta (NIT-1) cell lines to understand the role and differential survival of these two predominant islet cell populations. A total of 56 proteins in NIT-1 cells and 50 in αTC-1 cells were differentially expressed when exposed to proinflammatory cytokines. The major difference in the protein expression between cytokine-treated NIT-1 and αTC-1 cells was free radical scavenging enzymes. A similar observation was made in cytokine-treated whole islets, where a comprehensive analysis of subcellular fractions revealed that 438 unique proteins were differentially expressed under inflammatory conditions. Our data indicate that beta cells are relatively susceptible to ER and oxidative stress and reveal key pathways that are dysregulated in beta cells during cytokine exposure. Additionally, in the islets, inflammation also leads to enhanced antigen presentation, which completes a three-way insult on beta cells, rendering them targets of infiltrating T lymphocytes. ",
keywords = "Animals, Blotting, Western, Diabetes Mellitus, Experimental/metabolism, Endoplasmic Reticulum/metabolism, Islets of Langerhans/metabolism, Mice, Mice, Inbred NOD, Oxidative Stress",
author = "Gorasia, {Dhana G} and Dudek, {Nadine L} and Veith, {Paul D} and Renu Shankar and Helena Safavi-Hemami and Williamson, {Nicholas A} and Reynolds, {Eric C} and Hubbard, {Michael J} and Purcell, {Anthony W}",
year = "2015",
doi = "10.1021/pr500643h",
language = "English",
volume = "14",
pages = "688--99",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "2",

}

RIS

TY - JOUR

T1 - Pancreatic beta cells are highly susceptible to oxidative and ER stresses during the development of diabetes

AU - Gorasia, Dhana G

AU - Dudek, Nadine L

AU - Veith, Paul D

AU - Shankar, Renu

AU - Safavi-Hemami, Helena

AU - Williamson, Nicholas A

AU - Reynolds, Eric C

AU - Hubbard, Michael J

AU - Purcell, Anthony W

PY - 2015

Y1 - 2015

N2 - The complex interplay of many cell types and the temporal heterogeneity of pancreatic islet composition obscure the direct role of resident alpha and beta cells in the development of Type 1 diabetes. Therefore, in addition to studying islets isolated from non-obese diabetic mice, we analyzed homogeneous cell populations of murine alpha (αTC-1) and beta (NIT-1) cell lines to understand the role and differential survival of these two predominant islet cell populations. A total of 56 proteins in NIT-1 cells and 50 in αTC-1 cells were differentially expressed when exposed to proinflammatory cytokines. The major difference in the protein expression between cytokine-treated NIT-1 and αTC-1 cells was free radical scavenging enzymes. A similar observation was made in cytokine-treated whole islets, where a comprehensive analysis of subcellular fractions revealed that 438 unique proteins were differentially expressed under inflammatory conditions. Our data indicate that beta cells are relatively susceptible to ER and oxidative stress and reveal key pathways that are dysregulated in beta cells during cytokine exposure. Additionally, in the islets, inflammation also leads to enhanced antigen presentation, which completes a three-way insult on beta cells, rendering them targets of infiltrating T lymphocytes.

AB - The complex interplay of many cell types and the temporal heterogeneity of pancreatic islet composition obscure the direct role of resident alpha and beta cells in the development of Type 1 diabetes. Therefore, in addition to studying islets isolated from non-obese diabetic mice, we analyzed homogeneous cell populations of murine alpha (αTC-1) and beta (NIT-1) cell lines to understand the role and differential survival of these two predominant islet cell populations. A total of 56 proteins in NIT-1 cells and 50 in αTC-1 cells were differentially expressed when exposed to proinflammatory cytokines. The major difference in the protein expression between cytokine-treated NIT-1 and αTC-1 cells was free radical scavenging enzymes. A similar observation was made in cytokine-treated whole islets, where a comprehensive analysis of subcellular fractions revealed that 438 unique proteins were differentially expressed under inflammatory conditions. Our data indicate that beta cells are relatively susceptible to ER and oxidative stress and reveal key pathways that are dysregulated in beta cells during cytokine exposure. Additionally, in the islets, inflammation also leads to enhanced antigen presentation, which completes a three-way insult on beta cells, rendering them targets of infiltrating T lymphocytes.

KW - Animals

KW - Blotting, Western

KW - Diabetes Mellitus, Experimental/metabolism

KW - Endoplasmic Reticulum/metabolism

KW - Islets of Langerhans/metabolism

KW - Mice

KW - Mice, Inbred NOD

KW - Oxidative Stress

U2 - 10.1021/pr500643h

DO - 10.1021/pr500643h

M3 - Journal article

C2 - 25412008

VL - 14

SP - 688

EP - 699

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 2

ER -

ID: 232824485