Occurrence, distribution and motor effects of galanin in the porcine lower esophageal sphincter
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Occurrence, distribution and motor effects of galanin in the porcine lower esophageal sphincter. / Holst, J J; Poulsen, Steen Seier.
I: Digestion, Bind 42, Nr. 3, 1989, s. 151-7.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Occurrence, distribution and motor effects of galanin in the porcine lower esophageal sphincter
AU - Holst, J J
AU - Poulsen, Steen Seier
PY - 1989
Y1 - 1989
N2 - Specimens from the lower esophageal sphincter (LES) region of the pig were analyzed for galanin-like immunoreactivity (GAL-LI) using radioimmunoassay and immunohistochemistry. The mean concentration of GAL-LI was 3.4 pmol/g tissue. GAL-LI nerve fibers were observed surrounding muscle bundles in the smooth circular muscle layer, and in ganglion cells of the myenteric plexus. Bolus injection of 40-200 pmol/kg of galanin directly into the arterial supply of the resting LES increased the LES pressure (LESP) dose-dependently, whereas slow infusion of 2-100 pmol galanin/kg/min had no effect on resting LESP. The vagally increased LESP was not modulated by simultaneous galanin infusion, and the concentration of galanin in the venous effluent of the LES declined insignificantly during vagal stimulation. It is concluded that galanin may be an important neuropeptide for the modulation of resting LES tone.
AB - Specimens from the lower esophageal sphincter (LES) region of the pig were analyzed for galanin-like immunoreactivity (GAL-LI) using radioimmunoassay and immunohistochemistry. The mean concentration of GAL-LI was 3.4 pmol/g tissue. GAL-LI nerve fibers were observed surrounding muscle bundles in the smooth circular muscle layer, and in ganglion cells of the myenteric plexus. Bolus injection of 40-200 pmol/kg of galanin directly into the arterial supply of the resting LES increased the LES pressure (LESP) dose-dependently, whereas slow infusion of 2-100 pmol galanin/kg/min had no effect on resting LESP. The vagally increased LESP was not modulated by simultaneous galanin infusion, and the concentration of galanin in the venous effluent of the LES declined insignificantly during vagal stimulation. It is concluded that galanin may be an important neuropeptide for the modulation of resting LES tone.
KW - Animals
KW - Electric Stimulation
KW - Esophagus
KW - Galanin
KW - Immunohistochemistry
KW - Movement
KW - Neuropeptides
KW - Osmolar Concentration
KW - Peptides
KW - Swine
KW - Tissue Distribution
KW - Vagus Nerve
M3 - Journal article
C2 - 2475382
VL - 42
SP - 151
EP - 157
JO - Digestion
JF - Digestion
SN - 0012-2823
IS - 3
ER -
ID: 47488259