New nanocomposites for SERS studies of living cells and mitochondria

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Standard

New nanocomposites for SERS studies of living cells and mitochondria. / Sarycheva, A. S.; Brazhe, N. A.; Baizhumanov, A. A.; Nikelshparg, E. I.; Semenova, A. A.; Garshev, A. V.; Baranchikov, A. E.; Ivanov, V. K.; Maksimov, G. V.; Sosnovtseva, O.; Goodilin, E. A.

I: Journal of Materials Chemistry B, Bind 4, Nr. 3, 2016, s. 539-546.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Sarycheva, AS, Brazhe, NA, Baizhumanov, AA, Nikelshparg, EI, Semenova, AA, Garshev, AV, Baranchikov, AE, Ivanov, VK, Maksimov, GV, Sosnovtseva, O & Goodilin, EA 2016, 'New nanocomposites for SERS studies of living cells and mitochondria', Journal of Materials Chemistry B, bind 4, nr. 3, s. 539-546. https://doi.org/10.1039/c5tb01886b

APA

Sarycheva, A. S., Brazhe, N. A., Baizhumanov, A. A., Nikelshparg, E. I., Semenova, A. A., Garshev, A. V., Baranchikov, A. E., Ivanov, V. K., Maksimov, G. V., Sosnovtseva, O., & Goodilin, E. A. (2016). New nanocomposites for SERS studies of living cells and mitochondria. Journal of Materials Chemistry B, 4(3), 539-546. https://doi.org/10.1039/c5tb01886b

Vancouver

Sarycheva AS, Brazhe NA, Baizhumanov AA, Nikelshparg EI, Semenova AA, Garshev AV o.a. New nanocomposites for SERS studies of living cells and mitochondria. Journal of Materials Chemistry B. 2016;4(3):539-546. https://doi.org/10.1039/c5tb01886b

Author

Sarycheva, A. S. ; Brazhe, N. A. ; Baizhumanov, A. A. ; Nikelshparg, E. I. ; Semenova, A. A. ; Garshev, A. V. ; Baranchikov, A. E. ; Ivanov, V. K. ; Maksimov, G. V. ; Sosnovtseva, O. ; Goodilin, E. A. / New nanocomposites for SERS studies of living cells and mitochondria. I: Journal of Materials Chemistry B. 2016 ; Bind 4, Nr. 3. s. 539-546.

Bibtex

@article{87d8559a45ca4403b03f2d08b45201d8,
title = "New nanocomposites for SERS studies of living cells and mitochondria",
abstract = "A great enhancement in Raman scattering (SERS) from heme-containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver–silica beads prepared using a new method involving aerosol pyrolysis with aqueous diamminesilver(I) hydroxide as a unique source of plasmonic nanoparticles for SiO2 microspheres. The recorded SERS spectra reveal a set of characteristic peaks at 750, 1127, 1170, 1371, 1565, 1585 and 1638 cm−1, resulting from the normal group vibrations of the pyrrole rings, methine bridges and side radicals in the heme molecules. The SERS spectra of functional mitochondria are sensitive to the activity of the mitochondrial electron transport chain, thus making the method a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites are highly suitable for the analysis of biological objects due to their robust synthesis and superior spatial and temporal signal reproducibility, which was preserved for a period of at least one year.",
author = "Sarycheva, {A. S.} and Brazhe, {N. A.} and Baizhumanov, {A. A.} and Nikelshparg, {E. I.} and Semenova, {A. A.} and Garshev, {A. V.} and Baranchikov, {A. E.} and Ivanov, {V. K.} and Maksimov, {G. V.} and O. Sosnovtseva and Goodilin, {E. A.}",
year = "2016",
doi = "10.1039/c5tb01886b",
language = "English",
volume = "4",
pages = "539--546",
journal = "Journal of Materials Chemistry B",
issn = "2050-750X",
publisher = "Royal Society of Chemistry",
number = "3",

}

RIS

TY - JOUR

T1 - New nanocomposites for SERS studies of living cells and mitochondria

AU - Sarycheva, A. S.

AU - Brazhe, N. A.

AU - Baizhumanov, A. A.

AU - Nikelshparg, E. I.

AU - Semenova, A. A.

AU - Garshev, A. V.

AU - Baranchikov, A. E.

AU - Ivanov, V. K.

AU - Maksimov, G. V.

AU - Sosnovtseva, O.

AU - Goodilin, E. A.

PY - 2016

Y1 - 2016

N2 - A great enhancement in Raman scattering (SERS) from heme-containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver–silica beads prepared using a new method involving aerosol pyrolysis with aqueous diamminesilver(I) hydroxide as a unique source of plasmonic nanoparticles for SiO2 microspheres. The recorded SERS spectra reveal a set of characteristic peaks at 750, 1127, 1170, 1371, 1565, 1585 and 1638 cm−1, resulting from the normal group vibrations of the pyrrole rings, methine bridges and side radicals in the heme molecules. The SERS spectra of functional mitochondria are sensitive to the activity of the mitochondrial electron transport chain, thus making the method a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites are highly suitable for the analysis of biological objects due to their robust synthesis and superior spatial and temporal signal reproducibility, which was preserved for a period of at least one year.

AB - A great enhancement in Raman scattering (SERS) from heme-containing submembrane biomolecules inside intact erythrocytes and functional mitochondria is demonstrated for the first time using silver–silica beads prepared using a new method involving aerosol pyrolysis with aqueous diamminesilver(I) hydroxide as a unique source of plasmonic nanoparticles for SiO2 microspheres. The recorded SERS spectra reveal a set of characteristic peaks at 750, 1127, 1170, 1371, 1565, 1585 and 1638 cm−1, resulting from the normal group vibrations of the pyrrole rings, methine bridges and side radicals in the heme molecules. The SERS spectra of functional mitochondria are sensitive to the activity of the mitochondrial electron transport chain, thus making the method a novel label-free approach to monitor the redox state and conformation of cytochromes in their natural cell environment. The developed nanocomposites are highly suitable for the analysis of biological objects due to their robust synthesis and superior spatial and temporal signal reproducibility, which was preserved for a period of at least one year.

U2 - 10.1039/c5tb01886b

DO - 10.1039/c5tb01886b

M3 - Journal article

VL - 4

SP - 539

EP - 546

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

SN - 2050-750X

IS - 3

ER -

ID: 154520087