Mechanisms of pancreatic islet cell destruction. Dose-dependent cytotoxic effect of soluble blood mononuclear cell mediators on isolated islets of Langerhans
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Mechanisms of pancreatic islet cell destruction. Dose-dependent cytotoxic effect of soluble blood mononuclear cell mediators on isolated islets of Langerhans. / Mandrup-Poulsen, T; Bendtzen, K; Nerup, J; Egeberg, J; Nielsen, Jens Høiriis.
I: Allergy, Bind 41, Nr. 4, 05.1986, s. 250-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Mechanisms of pancreatic islet cell destruction. Dose-dependent cytotoxic effect of soluble blood mononuclear cell mediators on isolated islets of Langerhans
AU - Mandrup-Poulsen, T
AU - Bendtzen, K
AU - Nerup, J
AU - Egeberg, J
AU - Nielsen, Jens Høiriis
PY - 1986/5
Y1 - 1986/5
N2 - Supernatants of peripheral blood mononuclear cells from healthy human donors stimulated with recall antigen (purified protein derivative of tuberculin) or lectin (phytohaemagglutinin) markedly inhibited the insulin release from isolated human and rat islets of Langerhans, and decreased rat islet contents of insulin and glucagon in a dose-dependent manner. A maximal effect on islet function was obtained with supernatant concentrations down to 5%. Supernatants of mononuclear cells stimulated with tuberculin were more potent than supernatants produced by lectin stimulation. Culture medium reconstituted with tuberculin or phytohaemagglutinin did not impair islet function. Electron microscopy demonstrated that supernatants were cytotoxic to islet cells. The cytotoxic mononuclear cell mediator(s) was non-dialysable, sensitive to heating to 56 degrees C, labile even when stored at -70 degrees C, but stable when lyophilised.
AB - Supernatants of peripheral blood mononuclear cells from healthy human donors stimulated with recall antigen (purified protein derivative of tuberculin) or lectin (phytohaemagglutinin) markedly inhibited the insulin release from isolated human and rat islets of Langerhans, and decreased rat islet contents of insulin and glucagon in a dose-dependent manner. A maximal effect on islet function was obtained with supernatant concentrations down to 5%. Supernatants of mononuclear cells stimulated with tuberculin were more potent than supernatants produced by lectin stimulation. Culture medium reconstituted with tuberculin or phytohaemagglutinin did not impair islet function. Electron microscopy demonstrated that supernatants were cytotoxic to islet cells. The cytotoxic mononuclear cell mediator(s) was non-dialysable, sensitive to heating to 56 degrees C, labile even when stored at -70 degrees C, but stable when lyophilised.
KW - Animals
KW - Autoimmune Diseases
KW - Biological Agents
KW - Cytokines
KW - Cytotoxicity, Immunologic
KW - Diabetes Mellitus, Type 1
KW - Dose-Response Relationship, Immunologic
KW - Humans
KW - Insulin
KW - Islets of Langerhans
KW - Leukocytes
KW - Male
KW - Phytohemagglutinins
KW - Rats
KW - Rats, Inbred Strains
KW - Tuberculin
M3 - Journal article
C2 - 3530029
VL - 41
SP - 250
EP - 259
JO - Allergy: European Journal of Allergy and Clinical Immunology
JF - Allergy: European Journal of Allergy and Clinical Immunology
SN - 0105-4538
IS - 4
ER -
ID: 47975060