Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells

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Standard

Mechanisms of pancreatic beta-cell growth and regeneration : studies on rat insulinoma cells. / Nielsen, Jens Høiriis.

I: Experimental and Clinical Endocrinology and Diabetes. Supplement, Bind 93, Nr. 2-3, 1989, s. 277-85.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Nielsen, JH 1989, 'Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells', Experimental and Clinical Endocrinology and Diabetes. Supplement, bind 93, nr. 2-3, s. 277-85.

APA

Nielsen, J. H. (1989). Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells. Experimental and Clinical Endocrinology and Diabetes. Supplement, 93(2-3), 277-85.

Vancouver

Nielsen JH. Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells. Experimental and Clinical Endocrinology and Diabetes. Supplement. 1989;93(2-3):277-85.

Author

Nielsen, Jens Høiriis. / Mechanisms of pancreatic beta-cell growth and regeneration : studies on rat insulinoma cells. I: Experimental and Clinical Endocrinology and Diabetes. Supplement. 1989 ; Bind 93, Nr. 2-3. s. 277-85.

Bibtex

@article{17ac22c99aa9452bba7e322b68ad6ed9,
title = "Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells",
abstract = "Information about the mechanism of beta-cell growth and regeneration may be obtained by studies of insulinoma cells. In the present study the growth and function of the rat insulinoma cell lines RINm5F and 5AH were evaluated by addition of serum, hormones, and growth factors. It was found that transferrin is the only obligatory factor whereas growth hormone, epidermal growth factor, fibroblast growth factor, and TRH had modulating effects. A heat-labile heparin binding serum factor which stimulated thymidine incorporation but not cell proliferation was demonstrated in human serum. Measurements of insulin mRNA content showed that the insulinoma cells only contained about 2{\%} of that of normal rat beta-cells. These results are discussed in relation to the role of growth factors, oncogenes, and differentiation in the growth and regeneration of beta-cells.",
keywords = "Adenoma, Islet Cell, Animals, Cell Division, Cell Line, Transformed, Cell Transformation, Neoplastic, Epidermal Growth Factor, Fibroblast Growth Factors, Growth Hormone, Insulin, Insulinoma, Islets of Langerhans, Pancreas, Pancreatic Neoplasms, RNA, Messenger, Rats, Regeneration, Thymidine, Thyrotropin-Releasing Hormone, Transferrin, Tumor Cells, Cultured",
author = "Nielsen, {Jens H{\o}iriis}",
year = "1989",
language = "English",
volume = "93",
pages = "277--85",
journal = "Experimental and Clinical Endocrinology and Diabetes. Supplement",
issn = "0948-146X",
publisher = "Johann Ambrosius Barth Verlag in Medizinverlage Heidelberg GmbH & Co. KG",
number = "2-3",

}

RIS

TY - JOUR

T1 - Mechanisms of pancreatic beta-cell growth and regeneration

T2 - studies on rat insulinoma cells

AU - Nielsen, Jens Høiriis

PY - 1989

Y1 - 1989

N2 - Information about the mechanism of beta-cell growth and regeneration may be obtained by studies of insulinoma cells. In the present study the growth and function of the rat insulinoma cell lines RINm5F and 5AH were evaluated by addition of serum, hormones, and growth factors. It was found that transferrin is the only obligatory factor whereas growth hormone, epidermal growth factor, fibroblast growth factor, and TRH had modulating effects. A heat-labile heparin binding serum factor which stimulated thymidine incorporation but not cell proliferation was demonstrated in human serum. Measurements of insulin mRNA content showed that the insulinoma cells only contained about 2% of that of normal rat beta-cells. These results are discussed in relation to the role of growth factors, oncogenes, and differentiation in the growth and regeneration of beta-cells.

AB - Information about the mechanism of beta-cell growth and regeneration may be obtained by studies of insulinoma cells. In the present study the growth and function of the rat insulinoma cell lines RINm5F and 5AH were evaluated by addition of serum, hormones, and growth factors. It was found that transferrin is the only obligatory factor whereas growth hormone, epidermal growth factor, fibroblast growth factor, and TRH had modulating effects. A heat-labile heparin binding serum factor which stimulated thymidine incorporation but not cell proliferation was demonstrated in human serum. Measurements of insulin mRNA content showed that the insulinoma cells only contained about 2% of that of normal rat beta-cells. These results are discussed in relation to the role of growth factors, oncogenes, and differentiation in the growth and regeneration of beta-cells.

KW - Adenoma, Islet Cell

KW - Animals

KW - Cell Division

KW - Cell Line, Transformed

KW - Cell Transformation, Neoplastic

KW - Epidermal Growth Factor

KW - Fibroblast Growth Factors

KW - Growth Hormone

KW - Insulin

KW - Insulinoma

KW - Islets of Langerhans

KW - Pancreas

KW - Pancreatic Neoplasms

KW - RNA, Messenger

KW - Rats

KW - Regeneration

KW - Thymidine

KW - Thyrotropin-Releasing Hormone

KW - Transferrin

KW - Tumor Cells, Cultured

M3 - Journal article

C2 - 2550270

VL - 93

SP - 277

EP - 285

JO - Experimental and Clinical Endocrinology and Diabetes. Supplement

JF - Experimental and Clinical Endocrinology and Diabetes. Supplement

SN - 0948-146X

IS - 2-3

ER -

ID: 47974326