MCPIP1 is a novel link between diabetogenic conditions and impaired insulin secretory capacity
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MCPIP1 is a novel link between diabetogenic conditions and impaired insulin secretory capacity. / Tyka, Karolina; Joerns, Anne; Dunst, Alessia; Tang, Yadi; Bryde, Tenna Holgersen; Mehmeti, Ilir; Walentinsson, Anna; Marselli, Lorella; Cnop, Miriam; Tyrberg, Bjorn; Marzec, Michal T.; Gurgul-Convey, Ewa.
I: B B A - Molecular Basis of Disease, Bind 1867, Nr. 10, 166199, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - MCPIP1 is a novel link between diabetogenic conditions and impaired insulin secretory capacity
AU - Tyka, Karolina
AU - Joerns, Anne
AU - Dunst, Alessia
AU - Tang, Yadi
AU - Bryde, Tenna Holgersen
AU - Mehmeti, Ilir
AU - Walentinsson, Anna
AU - Marselli, Lorella
AU - Cnop, Miriam
AU - Tyrberg, Bjorn
AU - Marzec, Michal T.
AU - Gurgul-Convey, Ewa
PY - 2021
Y1 - 2021
N2 - During diabetes development insulin production and glucose-stimulated insulin secretion (GSIS) are defective due to inflammation-related, yet not fully understood mechanisms. MCPIP1 (monocyte chemotactic protein-induced protein-1) is a strong regulator of inflammation, and acts predominantly as a specific RNase. The impact of MCPIP1 on insulin secretory capacity is unknown.We show that the expression of the ZC3H12A gene, which encodes MCPIP1, was induced by T1DM- and by T2DM-simulating conditions, with a stronger effect of cytokines. The number of MCPIP1-positive pancreatic islet-cells, including beta-cells, was significantly higher in diabetic compared to nondiabetic individuals. In the 3' UTR regions of mRNAs coding for Pdx1 (pancreatic and duodenal homeobox 1), FoxO1 (forkhead box protein O1), and of a novel regulator of insulin handling, Grp94 (glucose-regulated protein 94), MCPIP1-target structures were detected. Overexpression of the wild type MCPIP1(wt), but not of the mutant MCPIP1(D141N) (lacking the RNase activity), decreased the expression of genes involved in insulin production and GSIS. Additionally INS1-E-MCPIP1(wt) cells exhibited a higher Ire1 (inositol-requiring enzyme 1) expression. MCPIP1(wt) overexpression blunted GSIS and glucose-mediated calcium influx with no deleterious effects on glucose uptake or glucokinase activity.We identify MCPIP1 as a new common link between diabetogenic conditions and beta-cell failure. MCPIP1 may serve as an interesting target for novel beta-cell protective approaches.
AB - During diabetes development insulin production and glucose-stimulated insulin secretion (GSIS) are defective due to inflammation-related, yet not fully understood mechanisms. MCPIP1 (monocyte chemotactic protein-induced protein-1) is a strong regulator of inflammation, and acts predominantly as a specific RNase. The impact of MCPIP1 on insulin secretory capacity is unknown.We show that the expression of the ZC3H12A gene, which encodes MCPIP1, was induced by T1DM- and by T2DM-simulating conditions, with a stronger effect of cytokines. The number of MCPIP1-positive pancreatic islet-cells, including beta-cells, was significantly higher in diabetic compared to nondiabetic individuals. In the 3' UTR regions of mRNAs coding for Pdx1 (pancreatic and duodenal homeobox 1), FoxO1 (forkhead box protein O1), and of a novel regulator of insulin handling, Grp94 (glucose-regulated protein 94), MCPIP1-target structures were detected. Overexpression of the wild type MCPIP1(wt), but not of the mutant MCPIP1(D141N) (lacking the RNase activity), decreased the expression of genes involved in insulin production and GSIS. Additionally INS1-E-MCPIP1(wt) cells exhibited a higher Ire1 (inositol-requiring enzyme 1) expression. MCPIP1(wt) overexpression blunted GSIS and glucose-mediated calcium influx with no deleterious effects on glucose uptake or glucokinase activity.We identify MCPIP1 as a new common link between diabetogenic conditions and beta-cell failure. MCPIP1 may serve as an interesting target for novel beta-cell protective approaches.
KW - MCPIP1
KW - Diabetes
KW - Beta-cells
KW - Human islets
KW - Insulin production and secretion
KW - Inflammation
KW - NF-KAPPA-B
KW - INFLAMMATION
KW - CELLS
KW - DEGRADATION
KW - PROTEIN
KW - TYPE-1
KW - GENES
KW - RNASE
U2 - 10.1016/j.bbadis.2021.166199
DO - 10.1016/j.bbadis.2021.166199
M3 - Journal article
C2 - 34144091
VL - 1867
JO - B B A - Molecular Basis of Disease
JF - B B A - Molecular Basis of Disease
SN - 0925-4439
IS - 10
M1 - 166199
ER -
ID: 275529237