Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia
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Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia. / Nielsen, Mette M B; Lambertsen, Kate L; Clausen, Bettina H; Meyer, Morten; Bhandari, Dhaka R; Larsen, Søren T; Poulsen, Steen S; Spengler, Bernhard; Janfelt, Christian; Hansen, Harald S.
I: Scientific Reports, Bind 6, 39571, 22.12.2016, s. 1-14.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Mass spectrometry imaging of biomarker lipids for phagocytosis and signalling during focal cerebral ischaemia
AU - Nielsen, Mette M B
AU - Lambertsen, Kate L
AU - Clausen, Bettina H
AU - Meyer, Morten
AU - Bhandari, Dhaka R
AU - Larsen, Søren T
AU - Poulsen, Steen S
AU - Spengler, Bernhard
AU - Janfelt, Christian
AU - Hansen, Harald S
PY - 2016/12/22
Y1 - 2016/12/22
N2 - Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.
AB - Focal cerebral ischaemia has an initial phase of inflammation and tissue injury followed by a later phase of resolution and repair. Mass spectrometry imaging (desorption electrospray ionization and matrix assisted laser desorption ionization) was applied on brain sections from mice 2 h, 24 h, 5d, 7d, and 20d after permanent focal cerebral ischaemia. Within 24 h, N-acyl-phosphatidylethanolamines, lysophosphatidylcholine, and ceramide accumulated, while sphingomyelin disappeared. At the later resolution stages, bis(monoacylglycero)phosphate (BMP(22:6/22:6)), 2-arachidonoyl-glycerol, ceramide-phosphate, sphingosine-1-phosphate, lysophosphatidylserine, and cholesteryl ester appeared. At day 5 to 7, dihydroxy derivates of docosahexaenoic and docosapentaenoic acid, some of which may be pro-resolving mediators, e.g. resolvins, were found in the injured area, and BMP(22:6/22:6) co-localized with the macrophage biomarker CD11b, and probably with cholesteryl ester. Mass spectrometry imaging can visualize spatiotemporal changes in the lipidome during the progression and resolution of focal cerebral inflammation and suggests that BMP(22:6/22:6) and N-acyl-phosphatidylethanolamines can be used as biomarkers for phagocytizing macrophages/microglia cells and dead neurones, respectively.
U2 - 10.1038/srep39571
DO - 10.1038/srep39571
M3 - Journal article
C2 - 28004822
VL - 6
SP - 1
EP - 14
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
M1 - 39571
ER -
ID: 171548750