Localization in the gastrointestinal tract of immunoreactive prosomatostatin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Localization in the gastrointestinal tract of immunoreactive prosomatostatin. / Holst, J J; Poulsen, Steen Seier.

I: Regulatory Peptides, Bind 19, Nr. 3-4, 11.1987, s. 183-95.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Holst, JJ & Poulsen, SS 1987, 'Localization in the gastrointestinal tract of immunoreactive prosomatostatin', Regulatory Peptides, bind 19, nr. 3-4, s. 183-95.

APA

Holst, J. J., & Poulsen, S. S. (1987). Localization in the gastrointestinal tract of immunoreactive prosomatostatin. Regulatory Peptides, 19(3-4), 183-95.

Vancouver

Holst JJ, Poulsen SS. Localization in the gastrointestinal tract of immunoreactive prosomatostatin. Regulatory Peptides. 1987 nov.;19(3-4):183-95.

Author

Holst, J J ; Poulsen, Steen Seier. / Localization in the gastrointestinal tract of immunoreactive prosomatostatin. I: Regulatory Peptides. 1987 ; Bind 19, Nr. 3-4. s. 183-95.

Bibtex

@article{f5719e85f4124bf897655c915f628f6e,
title = "Localization in the gastrointestinal tract of immunoreactive prosomatostatin",
abstract = "Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.",
keywords = "Animals, Chromatography, Gel, Colon, Digestive System, Duodenum, Ileum, Immunohistochemistry, Jejunum, Protein Precursors, Radioimmunoassay, Somatostatin, Stomach, Swine",
author = "Holst, {J J} and Poulsen, {Steen Seier}",
year = "1987",
month = nov,
language = "English",
volume = "19",
pages = "183--95",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "3-4",

}

RIS

TY - JOUR

T1 - Localization in the gastrointestinal tract of immunoreactive prosomatostatin

AU - Holst, J J

AU - Poulsen, Steen Seier

PY - 1987/11

Y1 - 1987/11

N2 - Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.

AB - Antisera against 5 different regions of the entire prosomatostatin molecule were used for immunohistochemical mapping of prosomatostatin-containing structures in the pig gastrointestinal tract, and for radioimmunological and chromatographical analysis of the products of prosomatostatin in extracts of ileal mucosa. The latter showed that the antisera were capable of identifying components containing N-terminal as well as C-terminal parts of prosomatostatin. Endocrine cells were identified with all antisera in most parts of the gastrointestinal tract, and varicose nerve fibres were observed in all parts of the small intestine but not in the stomach and the colon. The colon contained very few immunoreactive structures. Immunoreactive nerve cell bodies were found in the submucous plexus of the small intestine. All immunoreactive endocrine cells in the stomach and the duodenum and all immunoreactive nerves were stained by all 5 antisera whereas the small intestinal endocrine cells did not stain for the most N-terminal region of prosomatostatin. The results suggest that all gastrointestinal somatostatin is derived from the same precursor molecule, which, however, in the small intestinal endocrine cells is processed differently from that of the other tissues.

KW - Animals

KW - Chromatography, Gel

KW - Colon

KW - Digestive System

KW - Duodenum

KW - Ileum

KW - Immunohistochemistry

KW - Jejunum

KW - Protein Precursors

KW - Radioimmunoassay

KW - Somatostatin

KW - Stomach

KW - Swine

M3 - Journal article

C2 - 2893436

VL - 19

SP - 183

EP - 195

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 3-4

ER -

ID: 47488519