Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

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Standard

Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. / Bensimon-Brito, Anabela; Boezio, Giulia L M; Cardeira-da-Silva, João; Wietelmann, Astrid; Ramkumar, Srinath; Lundegaard, Pia R; Helker, Christian S M; Ramadass, Radhan; Piesker, Janett; Nauerth, Arno; Mueller, Clemens; Stainier, Didier Y R.

I: Cardiovascular Research, Bind 118, Nr. 12, 2022, s. 2665–2687.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Bensimon-Brito, A, Boezio, GLM, Cardeira-da-Silva, J, Wietelmann, A, Ramkumar, S, Lundegaard, PR, Helker, CSM, Ramadass, R, Piesker, J, Nauerth, A, Mueller, C & Stainier, DYR 2022, 'Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish', Cardiovascular Research, bind 118, nr. 12, s. 2665–2687. https://doi.org/10.1093/cvr/cvab310

APA

Bensimon-Brito, A., Boezio, G. L. M., Cardeira-da-Silva, J., Wietelmann, A., Ramkumar, S., Lundegaard, P. R., Helker, C. S. M., Ramadass, R., Piesker, J., Nauerth, A., Mueller, C., & Stainier, D. Y. R. (2022). Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. Cardiovascular Research, 118(12), 2665–2687. https://doi.org/10.1093/cvr/cvab310

Vancouver

Bensimon-Brito A, Boezio GLM, Cardeira-da-Silva J, Wietelmann A, Ramkumar S, Lundegaard PR o.a. Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. Cardiovascular Research. 2022;118(12):2665–2687. https://doi.org/10.1093/cvr/cvab310

Author

Bensimon-Brito, Anabela ; Boezio, Giulia L M ; Cardeira-da-Silva, João ; Wietelmann, Astrid ; Ramkumar, Srinath ; Lundegaard, Pia R ; Helker, Christian S M ; Ramadass, Radhan ; Piesker, Janett ; Nauerth, Arno ; Mueller, Clemens ; Stainier, Didier Y R. / Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. I: Cardiovascular Research. 2022 ; Bind 118, Nr. 12. s. 2665–2687.

Bibtex

@article{b9c5e4ef7e5d484caa2909e0a0298f13,

title = "Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish",

abstract = "AIMS: Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque.METHODS AND RESULTS: Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyze cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically.CONCLUSION: We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.TRANSLATIONAL PERSPECTIVE: This study further illustrates the importance of the zebrafish model to investigate cardiovascular phenotypes including morphological and functional alterations as observed in human disease settings.",

author = "Anabela Bensimon-Brito and Boezio, {Giulia L M} and Jo{\~a}o Cardeira-da-Silva and Astrid Wietelmann and Srinath Ramkumar and Lundegaard, {Pia R} and Helker, {Christian S M} and Radhan Ramadass and Janett Piesker and Arno Nauerth and Clemens Mueller and Stainier, {Didier Y R}",

note = "{\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of the European Society of Cardiology.",

year = "2022",

doi = "10.1093/cvr/cvab310",

language = "English",

volume = "118",

pages = "2665–2687",

journal = "Cardiovascular Research",

issn = "0008-6363",

publisher = "Oxford University Press",

number = "12",

}

RIS

TY - JOUR

T1 - Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

AU - Bensimon-Brito, Anabela

AU - Boezio, Giulia L M

AU - Cardeira-da-Silva, João

AU - Wietelmann, Astrid

AU - Ramkumar, Srinath

AU - Lundegaard, Pia R

AU - Helker, Christian S M

AU - Ramadass, Radhan

AU - Piesker, Janett

AU - Nauerth, Arno

AU - Mueller, Clemens

AU - Stainier, Didier Y R

PY - 2022

Y1 - 2022

N2 - AIMS: Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque.METHODS AND RESULTS: Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyze cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically.CONCLUSION: We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.TRANSLATIONAL PERSPECTIVE: This study further illustrates the importance of the zebrafish model to investigate cardiovascular phenotypes including morphological and functional alterations as observed in human disease settings.

AB - AIMS: Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque.METHODS AND RESULTS: Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyze cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between hemodynamic defects and structural alterations of the heart, as observed clinically.CONCLUSION: We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.TRANSLATIONAL PERSPECTIVE: This study further illustrates the importance of the zebrafish model to investigate cardiovascular phenotypes including morphological and functional alterations as observed in human disease settings.

U2 - 10.1093/cvr/cvab310

DO - 10.1093/cvr/cvab310

M3 - Journal article

C2 - 34609500

VL - 118

SP - 2665

EP - 2687

JO - Cardiovascular Research

JF - Cardiovascular Research

SN - 0008-6363

IS - 12

ER -

ID: 285874327

Biomedicinsk Institut