Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy

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Standard

Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy. / Nalla, Amarnadh; Ringholm, Lene; Søstrup, Birgitte; Højrup, Peter; Thim, Lars; Levery, Steven B; Vakhrushev, Sergey Y; Billestrup, Nils; Mathiesen, Elisabeth R; Damm, Peter; Nielsen, Jens H.

I: Acta Obstetricia et Gynecologica Scandinavica, Bind 93, Nr. 11, 11.2014, s. 1181-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Nalla, A, Ringholm, L, Søstrup, B, Højrup, P, Thim, L, Levery, SB, Vakhrushev, SY, Billestrup, N, Mathiesen, ER, Damm, P & Nielsen, JH 2014, 'Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy', Acta Obstetricia et Gynecologica Scandinavica, bind 93, nr. 11, s. 1181-9. https://doi.org/10.1111/aogs.12505

APA

Nalla, A., Ringholm, L., Søstrup, B., Højrup, P., Thim, L., Levery, S. B., ... Nielsen, J. H. (2014). Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy. Acta Obstetricia et Gynecologica Scandinavica, 93(11), 1181-9. https://doi.org/10.1111/aogs.12505

Vancouver

Nalla A, Ringholm L, Søstrup B, Højrup P, Thim L, Levery SB o.a. Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy. Acta Obstetricia et Gynecologica Scandinavica. 2014 nov;93(11):1181-9. https://doi.org/10.1111/aogs.12505

Author

Nalla, Amarnadh ; Ringholm, Lene ; Søstrup, Birgitte ; Højrup, Peter ; Thim, Lars ; Levery, Steven B ; Vakhrushev, Sergey Y ; Billestrup, Nils ; Mathiesen, Elisabeth R ; Damm, Peter ; Nielsen, Jens H. / Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy. I: Acta Obstetricia et Gynecologica Scandinavica. 2014 ; Bind 93, Nr. 11. s. 1181-9.

Bibtex

@article{251c23c53ccb4dea92b12b2c336c6815,
title = "Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy",
abstract = "OBJECTIVE: Several studies have shown an increase in beta cell mass during pregnancy. Somatolactogenic hormones are known to stimulate the proliferation of existing beta cells in rodents whereas the mechanism in humans is still unclear. We hypothesize that in addition to somatolactogenic hormones there are other circulating factors involved in beta cell adaptation to pregnancy. This study aimed at screening for potential pregnancy-associated circulating beta cell growth factors.SAMPLES: Serum samples from nonpregnant and pregnant women.METHODS: The effect of serum from pregnant women on the proliferation of rat beta cells was studied using [3H]thymidine incorporation and 5-ethynyl-2'-deoxyuridine proliferation assays. In addition, serum from pregnant and nonpregnant women was fractionated by gel filtration and high performance liquid chromatography. The fractionated serum was screened for mitogenic activity in INS-1E cells. Proteins and peptides in mitogenic active serum fractions were identified by amino acid sequencing and mass spectrometry.MAIN OUTCOME MEASURES: Presence of circulating beta cell proliferating factors.RESULTS: Late gestational pregnancy serum significantly increased proliferation of rat beta cells compared with early pregnancy and nonpregnancy. The mitogenic active serum fractions contained proteins and peptides derived from kininogen-1, fibrinogen-α, α1-antitrypsin, apolipoprotein-A1, placental lactogen, angiotensinogen and serum albumin.CONCLUSION: Pregnancy serum is able to stimulate proliferation of rat beta cells. We have identified several circulating factors that may contribute to beta cell adaptation to pregnancy. Further studies are needed to elucidate their possible role in glucose homeostasis in the mother and her offspring.",
keywords = "Adaptation, Physiological, Adult, Amino Acid Sequence, Angiotensinogen, Animals, Animals, Newborn, Apolipoprotein A-I, Biological Markers, Cell Proliferation, Cells, Cultured, Chromatography, Gel, Chromatography, High Pressure Liquid, Female, Fibrinogen, Humans, Insulin-Secreting Cells, Kininogens, Mass Spectrometry, Placental Lactogen, Pregnancy, Pregnancy Trimesters, Rats, Rats, Wistar, Serum Albumin, alpha 1-Antitrypsin",
author = "Amarnadh Nalla and Lene Ringholm and Birgitte S{\o}strup and Peter H{\o}jrup and Lars Thim and Levery, {Steven B} and Vakhrushev, {Sergey Y} and Nils Billestrup and Mathiesen, {Elisabeth R} and Peter Damm and Nielsen, {Jens H}",
note = "{\circledC} 2014 Nordic Federation of Societies of Obstetrics and Gynecology.",
year = "2014",
month = "11",
doi = "10.1111/aogs.12505",
language = "English",
volume = "93",
pages = "1181--9",
journal = "Acta Obstetricia et Gynecologica Scandinavica",
issn = "0001-6349",
publisher = "JohnWiley & Sons Ltd",
number = "11",

}

RIS

TY - JOUR

T1 - Implications for the offspring of circulating factors involved in beta cell adaptation in pregnancy

AU - Nalla, Amarnadh

AU - Ringholm, Lene

AU - Søstrup, Birgitte

AU - Højrup, Peter

AU - Thim, Lars

AU - Levery, Steven B

AU - Vakhrushev, Sergey Y

AU - Billestrup, Nils

AU - Mathiesen, Elisabeth R

AU - Damm, Peter

AU - Nielsen, Jens H

N1 - © 2014 Nordic Federation of Societies of Obstetrics and Gynecology.

PY - 2014/11

Y1 - 2014/11

N2 - OBJECTIVE: Several studies have shown an increase in beta cell mass during pregnancy. Somatolactogenic hormones are known to stimulate the proliferation of existing beta cells in rodents whereas the mechanism in humans is still unclear. We hypothesize that in addition to somatolactogenic hormones there are other circulating factors involved in beta cell adaptation to pregnancy. This study aimed at screening for potential pregnancy-associated circulating beta cell growth factors.SAMPLES: Serum samples from nonpregnant and pregnant women.METHODS: The effect of serum from pregnant women on the proliferation of rat beta cells was studied using [3H]thymidine incorporation and 5-ethynyl-2'-deoxyuridine proliferation assays. In addition, serum from pregnant and nonpregnant women was fractionated by gel filtration and high performance liquid chromatography. The fractionated serum was screened for mitogenic activity in INS-1E cells. Proteins and peptides in mitogenic active serum fractions were identified by amino acid sequencing and mass spectrometry.MAIN OUTCOME MEASURES: Presence of circulating beta cell proliferating factors.RESULTS: Late gestational pregnancy serum significantly increased proliferation of rat beta cells compared with early pregnancy and nonpregnancy. The mitogenic active serum fractions contained proteins and peptides derived from kininogen-1, fibrinogen-α, α1-antitrypsin, apolipoprotein-A1, placental lactogen, angiotensinogen and serum albumin.CONCLUSION: Pregnancy serum is able to stimulate proliferation of rat beta cells. We have identified several circulating factors that may contribute to beta cell adaptation to pregnancy. Further studies are needed to elucidate their possible role in glucose homeostasis in the mother and her offspring.

AB - OBJECTIVE: Several studies have shown an increase in beta cell mass during pregnancy. Somatolactogenic hormones are known to stimulate the proliferation of existing beta cells in rodents whereas the mechanism in humans is still unclear. We hypothesize that in addition to somatolactogenic hormones there are other circulating factors involved in beta cell adaptation to pregnancy. This study aimed at screening for potential pregnancy-associated circulating beta cell growth factors.SAMPLES: Serum samples from nonpregnant and pregnant women.METHODS: The effect of serum from pregnant women on the proliferation of rat beta cells was studied using [3H]thymidine incorporation and 5-ethynyl-2'-deoxyuridine proliferation assays. In addition, serum from pregnant and nonpregnant women was fractionated by gel filtration and high performance liquid chromatography. The fractionated serum was screened for mitogenic activity in INS-1E cells. Proteins and peptides in mitogenic active serum fractions were identified by amino acid sequencing and mass spectrometry.MAIN OUTCOME MEASURES: Presence of circulating beta cell proliferating factors.RESULTS: Late gestational pregnancy serum significantly increased proliferation of rat beta cells compared with early pregnancy and nonpregnancy. The mitogenic active serum fractions contained proteins and peptides derived from kininogen-1, fibrinogen-α, α1-antitrypsin, apolipoprotein-A1, placental lactogen, angiotensinogen and serum albumin.CONCLUSION: Pregnancy serum is able to stimulate proliferation of rat beta cells. We have identified several circulating factors that may contribute to beta cell adaptation to pregnancy. Further studies are needed to elucidate their possible role in glucose homeostasis in the mother and her offspring.

KW - Adaptation, Physiological

KW - Adult

KW - Amino Acid Sequence

KW - Angiotensinogen

KW - Animals

KW - Animals, Newborn

KW - Apolipoprotein A-I

KW - Biological Markers

KW - Cell Proliferation

KW - Cells, Cultured

KW - Chromatography, Gel

KW - Chromatography, High Pressure Liquid

KW - Female

KW - Fibrinogen

KW - Humans

KW - Insulin-Secreting Cells

KW - Kininogens

KW - Mass Spectrometry

KW - Placental Lactogen

KW - Pregnancy

KW - Pregnancy Trimesters

KW - Rats

KW - Rats, Wistar

KW - Serum Albumin

KW - alpha 1-Antitrypsin

U2 - 10.1111/aogs.12505

DO - 10.1111/aogs.12505

M3 - Journal article

VL - 93

SP - 1181

EP - 1189

JO - Acta Obstetricia et Gynecologica Scandinavica

JF - Acta Obstetricia et Gynecologica Scandinavica

SN - 0001-6349

IS - 11

ER -

ID: 132899552