TY - JOUR

T1 - Human skeletal muscle perilipin 2 and 3 expression varies with insulin sensitivity

AU - Vigelsø Hansen, Andreas

AU - Prats Gavalda, Clara

AU - Ploug, Thorkil

AU - Dela, Flemming

AU - Helge, Jørn Wulff

PY - 2013/5

Y1 - 2013/5

N2 - Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups; type 2 diabetes patients (T2D), impaired glu- cose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hy- perinsulinaemic clamp was performed. Muscle sam- ples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perilipin 5, ATGL, HSL, EL or LPL between the groups. Con- clusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied mark- edly with insulin sensitivity. This difference in perili- pin expression may indicate that the lipid droplet function and thus storage and release of fatty acid- vary with insulin sensitivity.

AB - Background: Impaired insulin sensitivity may partly arise from a dysregulated lipid metabolism in human skeletal muscle. This study investigates the expression levels of perilipin 2, 3, and 5, and four key lipases in human skeletal muscle from the subjects that exhibit a range from normal to very low insulin sensitivity. Methods: 25 middle aged male participants were matched for lean body mass and recruited into three groups; type 2 diabetes patients (T2D), impaired glu- cose tolerance (IGT), and healthy sedentary controls (CON) according to their glucose tolerance and VO2peak. A muscle biopsy was obtained from vastus lateralis, and a two-step sequential euglycaemic-hy- perinsulinaemic clamp was performed. Muscle sam- ples were analyzed by Western blot for expression of perilipin 2, 3, 5, adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), endothelial lipase (EL) and lipoprotein lipase (LPL). Results: Perilipin 3 expression was higher in T2D compared to CON. Perilipin 2 expression was higher in CON than T2D. We observed no difference in expression of perilipin 5, ATGL, HSL, EL or LPL between the groups. Con- clusions: In the present study the muscle perilipin 3 expression and perilipin 2 expression varied mark- edly with insulin sensitivity. This difference in perili- pin expression may indicate that the lipid droplet function and thus storage and release of fatty acid- vary with insulin sensitivity.

KW - Faculty of Health and Medical Sciences

KW - Lipid Metabolism

KW - Insulin Sensitivity

KW - Perilipin Proteins

KW - Lipolysis

KW - Human Skeletal Muscle

KW - Lipotoxicity

U2 - 10.4236/jbise.2013.65A010

DO - 10.4236/jbise.2013.65A010

M3 - Journal article

VL - 6

SP - 65

EP - 72

JO - Journal of Biomedical Science and Engineering

JF - Journal of Biomedical Science and Engineering

SN - 1937-6871

IS - 5A

ER -