Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo
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Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Nature Metabolism |
| Vol/bind | 4 |
| Udgave nummer | 6 |
| Sider (fra-til) | 651-662 |
| ISSN | 2522-5812 |
| DOI | |
| Status | Udgivet - 2022 |
Bibliografisk note
Funding Information:
We apologise to our colleagues for the many key papers we were unable to cite due to space limitations, combined with the breadth of coverage required in a Consensus Statement. We thank A. J. Kowaltowski for helpful comments on the manuscript. Work in the laboratory of M.P.M. is supported by a grant from the Medical Research Council UK (no. MC_UU_00015/3) and by a Wellcome Trust Investigator award (no. 220257/Z/20/Z). Work in the laboratories of H.B. and V.E.K. is supported by National Institutes of Health (NIH), USA (grant nos. AI145406, CA165065, CA266342, HL114453, AI156924, NS076511, AI156923, NS061817 and NS117000). K.J.A.D. was supported by grant no. ES003598 from the National Institute of Environmental Health Sciences of the US NIH, and by grant no. AG052374 from the National Institute on Aging of the US NIH. R.R. was supported by grants from Universidad de la República, Uruguay (nos. CSIC_2018 and EI_2020). Research in the laboratory of B.H. is supported by grants from the National Medical Research Council of Singapore, National University of Singapore, National Research Foundation, Singapore and the Tan Chin Tuan Foundation. C.J.C. is supported by NIH (nos. GM 79465, GM 139245 and ES 28096) and is a CIFAR Scholar. Work in the laboratory of S.T. is supported by JST CREST (grant no. JPMJCR19H4), JSPS Kakenhi (grant nos. JP16H06276[AdAMS], JP19H05462 and JP20H05502) and a Research Grant of the Princess Takamatsu Cancer Research Fund (no. 19-25126). Work in the laboratory of T.P.D. is supported by grants from the German Research Council (nos. DFG, TRR184 and SPP2306) and the European Research Council (no. 742039). Work in the laboratory of H.Y. is supported by grants from the National Natural Science Foundation of China (nos. 32030053 and 32150710522). Work in the laboratory of M.J.D. is supported by the Novo Nordisk Foundation (grant nos. NNF13OC0004294, NNF19OC0058493 and NNF20SA0064214). Work in the laboratory of N.-G.L. is supported by the Swedish Research Council (2015-00418), Swedish Cancer Foundation, the Knut and Alice Wallenberg foundation, European Research Council (Advanced Grant 2016-741366) and the Novo Nordisk Foundation. B.H. is grateful to Alvin Loo for raising the issue of dubious kits to measure ROS.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.
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