Growth hormone-releasing factor induces c-fos expression in cultured primary pituitary cells
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Growth hormone-releasing factor induces c-fos expression in cultured primary pituitary cells. / Billestrup, Nils; Mitchell, R L; Vale, W; Verma, I M.
I: Molecular endocrinology (Baltimore, Md.), Bind 1, Nr. 4, 04.1987, s. 300-5.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Growth hormone-releasing factor induces c-fos expression in cultured primary pituitary cells
AU - Billestrup, Nils
AU - Mitchell, R L
AU - Vale, W
AU - Verma, I M
PY - 1987/4
Y1 - 1987/4
N2 - GH-releasing factor (GRF) and somatostatin regulates the secretion and biosynthesis of GH as well as the proliferation of GH-producing cells. In order to further characterize the mitogenic effect of GRF, we studied the expression of the proto-oncogene c-fos in primary pituitary cells. Maximal induction of c-fos mRNA was observed 20-60 min after stimulation with 5 nM GRF, returning to basal levels after 2 h. Somatostatin-14 (5 nM) partially inhibited the GRF induced c-fos expression. Forskolin and phorbol 12, 13 dibutyrate induced c-fos gene in cultured primary pituitary cells with similar kinetics. Transcription of the fos gene was accompanied by biosynthesis of the fos protein. Indirect immunofluorescence using a fos specific antibody, showed exclusive nuclear localization of the fos protein. These data demonstrate that GRF and somatostatin, in addition to regulating GH secretion and somatotroph proliferation, can also regulate the expression of c-fos proto-oncogene in primary somatotrophs.
AB - GH-releasing factor (GRF) and somatostatin regulates the secretion and biosynthesis of GH as well as the proliferation of GH-producing cells. In order to further characterize the mitogenic effect of GRF, we studied the expression of the proto-oncogene c-fos in primary pituitary cells. Maximal induction of c-fos mRNA was observed 20-60 min after stimulation with 5 nM GRF, returning to basal levels after 2 h. Somatostatin-14 (5 nM) partially inhibited the GRF induced c-fos expression. Forskolin and phorbol 12, 13 dibutyrate induced c-fos gene in cultured primary pituitary cells with similar kinetics. Transcription of the fos gene was accompanied by biosynthesis of the fos protein. Indirect immunofluorescence using a fos specific antibody, showed exclusive nuclear localization of the fos protein. These data demonstrate that GRF and somatostatin, in addition to regulating GH secretion and somatotroph proliferation, can also regulate the expression of c-fos proto-oncogene in primary somatotrophs.
KW - Animals
KW - Cells, Cultured
KW - Growth Hormone-Releasing Hormone
KW - Kinetics
KW - Male
KW - Pituitary Gland, Anterior
KW - Proto-Oncogenes
KW - RNA, Messenger
KW - Rats
KW - Rats, Inbred Strains
KW - Transcription, Genetic
U2 - 10.1210/mend-1-4-300
DO - 10.1210/mend-1-4-300
M3 - Journal article
C2 - 3135482
VL - 1
SP - 300
EP - 305
JO - Molecular Endocrinology
JF - Molecular Endocrinology
SN - 0888-8809
IS - 4
ER -
ID: 132900816