GPR162 is a beta cell CART receptor

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GPR162 is a beta cell CART receptor. / Lindqvist, Andreas; Abels, Mia; Shcherbina, Liliya; Ngara, Mtakai; Kryvokhyzha, Dmytro; Chriett, Sabrina; Riva, Matteo; Fajul, Abul; Barghouth, Mohammad; Luan, Cheng; Eliasson, Lena; Larsen, Olav; Rosenkilde, Mette M.; Zhang, Enming; Renström, Erik; Wierup, Nils.

I: iScience, Bind 26, Nr. 12, 108416, 2023.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lindqvist, A, Abels, M, Shcherbina, L, Ngara, M, Kryvokhyzha, D, Chriett, S, Riva, M, Fajul, A, Barghouth, M, Luan, C, Eliasson, L, Larsen, O, Rosenkilde, MM, Zhang, E, Renström, E & Wierup, N 2023, 'GPR162 is a beta cell CART receptor', iScience, bind 26, nr. 12, 108416. https://doi.org/10.1016/j.isci.2023.108416

APA

Lindqvist, A., Abels, M., Shcherbina, L., Ngara, M., Kryvokhyzha, D., Chriett, S., Riva, M., Fajul, A., Barghouth, M., Luan, C., Eliasson, L., Larsen, O., Rosenkilde, M. M., Zhang, E., Renström, E., & Wierup, N. (2023). GPR162 is a beta cell CART receptor. iScience, 26(12), [108416]. https://doi.org/10.1016/j.isci.2023.108416

Vancouver

Lindqvist A, Abels M, Shcherbina L, Ngara M, Kryvokhyzha D, Chriett S o.a. GPR162 is a beta cell CART receptor. iScience. 2023;26(12). 108416. https://doi.org/10.1016/j.isci.2023.108416

Author

Lindqvist, Andreas ; Abels, Mia ; Shcherbina, Liliya ; Ngara, Mtakai ; Kryvokhyzha, Dmytro ; Chriett, Sabrina ; Riva, Matteo ; Fajul, Abul ; Barghouth, Mohammad ; Luan, Cheng ; Eliasson, Lena ; Larsen, Olav ; Rosenkilde, Mette M. ; Zhang, Enming ; Renström, Erik ; Wierup, Nils. / GPR162 is a beta cell CART receptor. I: iScience. 2023 ; Bind 26, Nr. 12.

Bibtex

@article{818eed1ea0e645628c0cafba80da9fe9,

title = "GPR162 is a beta cell CART receptor",

abstract = "Cocaine and amphetamine-regulated transcript (CART) is expressed in pancreatic islet cells and neuronal elements. We have previously established insulinotropic actions of CART in human and rodent islets. The receptor for CART in the pancreatic beta cells is unidentified. We used RNA sequencing of Cartpt knockdown (KD) INS-1 832/13 cells and identified GPR162 as the most Cartpt-regulated receptor. We therefore tested if GPR162 mediates the effects of CART in beta cells. Binding of CART to GPR162 was established using proximity ligation assay, radioactive binding, and co-immunoprecipitation, and KD of Gpr162 mRNA caused reduced binding. Gpr162 KD cells had blunted CARTp-induced exocytosis, and reduced CARTp-induced insulin secretion. Furthermore, we identified a hitherto undescribed GPR162-dependent role of CART as a regulator of cytoskeletal arrangement. Thus, our findings provide mechanistic insight into the effect of CART on insulin secretion and show that GPR162 is the CART receptor in beta cells.",

keywords = "Biological sciences, Endocrinology, Natural sciences, Physiology",

author = "Andreas Lindqvist and Mia Abels and Liliya Shcherbina and Mtakai Ngara and Dmytro Kryvokhyzha and Sabrina Chriett and Matteo Riva and Abul Fajul and Mohammad Barghouth and Cheng Luan and Lena Eliasson and Olav Larsen and Rosenkilde, {Mette M.} and Enming Zhang and Erik Renstr{\"o}m and Nils Wierup",

note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",

year = "2023",

doi = "10.1016/j.isci.2023.108416",

language = "English",

volume = "26",

journal = "iScience",

issn = "2589-0042",

publisher = "Elsevier",

number = "12",

}

RIS

TY - JOUR

T1 - GPR162 is a beta cell CART receptor

AU - Lindqvist, Andreas

AU - Abels, Mia

AU - Shcherbina, Liliya

AU - Ngara, Mtakai

AU - Kryvokhyzha, Dmytro

AU - Chriett, Sabrina

AU - Riva, Matteo

AU - Fajul, Abul

AU - Barghouth, Mohammad

AU - Luan, Cheng

AU - Eliasson, Lena

AU - Larsen, Olav

AU - Rosenkilde, Mette M.

AU - Zhang, Enming

AU - Renström, Erik

AU - Wierup, Nils

PY - 2023

Y1 - 2023

N2 - Cocaine and amphetamine-regulated transcript (CART) is expressed in pancreatic islet cells and neuronal elements. We have previously established insulinotropic actions of CART in human and rodent islets. The receptor for CART in the pancreatic beta cells is unidentified. We used RNA sequencing of Cartpt knockdown (KD) INS-1 832/13 cells and identified GPR162 as the most Cartpt-regulated receptor. We therefore tested if GPR162 mediates the effects of CART in beta cells. Binding of CART to GPR162 was established using proximity ligation assay, radioactive binding, and co-immunoprecipitation, and KD of Gpr162 mRNA caused reduced binding. Gpr162 KD cells had blunted CARTp-induced exocytosis, and reduced CARTp-induced insulin secretion. Furthermore, we identified a hitherto undescribed GPR162-dependent role of CART as a regulator of cytoskeletal arrangement. Thus, our findings provide mechanistic insight into the effect of CART on insulin secretion and show that GPR162 is the CART receptor in beta cells.

AB - Cocaine and amphetamine-regulated transcript (CART) is expressed in pancreatic islet cells and neuronal elements. We have previously established insulinotropic actions of CART in human and rodent islets. The receptor for CART in the pancreatic beta cells is unidentified. We used RNA sequencing of Cartpt knockdown (KD) INS-1 832/13 cells and identified GPR162 as the most Cartpt-regulated receptor. We therefore tested if GPR162 mediates the effects of CART in beta cells. Binding of CART to GPR162 was established using proximity ligation assay, radioactive binding, and co-immunoprecipitation, and KD of Gpr162 mRNA caused reduced binding. Gpr162 KD cells had blunted CARTp-induced exocytosis, and reduced CARTp-induced insulin secretion. Furthermore, we identified a hitherto undescribed GPR162-dependent role of CART as a regulator of cytoskeletal arrangement. Thus, our findings provide mechanistic insight into the effect of CART on insulin secretion and show that GPR162 is the CART receptor in beta cells.

KW - Biological sciences

KW - Endocrinology

KW - Natural sciences

KW - Physiology

UR - http://www.scopus.com/inward/record.url?scp=85177871704&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2023.108416

DO - 10.1016/j.isci.2023.108416

M3 - Journal article

C2 - 38077141

AN - SCOPUS:85177871704

VL - 26

JO - iScience

JF - iScience

SN - 2589-0042

IS - 12

M1 - 108416

ER -

ID: 375312571