Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction

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Standard

Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction. / Hoeeg, Cecilie; Ringgaard, Lars; Christensen, Esben; Follin, Bjarke; Bentsen, Simon; Ripa, Rasmus Sejersten; Kjaer, Andreas.

I: Current Protocols, Bind 1, Nr. 2, e40, 2021.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Hoeeg, C, Ringgaard, L, Christensen, E, Follin, B, Bentsen, S, Ripa, RS & Kjaer, A 2021, 'Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction', Current Protocols, bind 1, nr. 2, e40. https://doi.org/10.1002/cpz1.40

APA

Hoeeg, C., Ringgaard, L., Christensen, E., Follin, B., Bentsen, S., Ripa, R. S., & Kjaer, A. (2021). Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction. Current Protocols, 1(2), [e40]. https://doi.org/10.1002/cpz1.40

Vancouver

Hoeeg C, Ringgaard L, Christensen E, Follin B, Bentsen S, Ripa RS o.a. Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction. Current Protocols. 2021;1(2). e40. https://doi.org/10.1002/cpz1.40

Author

Hoeeg, Cecilie ; Ringgaard, Lars ; Christensen, Esben ; Follin, Bjarke ; Bentsen, Simon ; Ripa, Rasmus Sejersten ; Kjaer, Andreas. / Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction. I: Current Protocols. 2021 ; Bind 1, Nr. 2.

Bibtex

@article{ee345fa8d81a4843b1f2109194d41d45,
title = "Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction",
abstract = "Angiogenesis is involved in regeneration of cardiac tissue following acute myocardial infarction (MI), a disease often investigated in rat models. Therefore, the ability to thoroughly evaluate the angiogenic response following experimentally induced MI in rats, and distinguish it from inflammation, is desired. This would enable evaluation of the angiogenic potential of new therapeutics and improve knowledge on MI pathophysiology. Due to the complex response to MI involving multiple cell types and the limited selection of rat-specific antibodies, careful optimization is crucial to capture this complexity. Here, we present an 8-color flow cytometry-based multicolor panel that will enable quantification of the ongoing angiogenic response as well as characterize the cells involved. A detailed description of tissue preparation, immunostaining, and gating strategy is provided.",
keywords = "angiogenesis, flow cytometry, immune response, myocardial infarction, rat cardiac tissue, rat heart",
author = "Cecilie Hoeeg and Lars Ringgaard and Esben Christensen and Bjarke Follin and Simon Bentsen and Ripa, {Rasmus Sejersten} and Andreas Kjaer",
note = "Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC",
year = "2021",
doi = "10.1002/cpz1.40",
language = "English",
volume = "1",
journal = "Current Protocols",
issn = "2691-1299",
publisher = "Wiley",
number = "2",

}

RIS

TY - JOUR

T1 - Flow Cytometric Evaluation of the Ongoing Angiogenic Response in Rat Cardiac Tissue Following Myocardial Infarction

AU - Hoeeg, Cecilie

AU - Ringgaard, Lars

AU - Christensen, Esben

AU - Follin, Bjarke

AU - Bentsen, Simon

AU - Ripa, Rasmus Sejersten

AU - Kjaer, Andreas

N1 - Publisher Copyright: © 2021 Wiley Periodicals LLC

PY - 2021

Y1 - 2021

N2 - Angiogenesis is involved in regeneration of cardiac tissue following acute myocardial infarction (MI), a disease often investigated in rat models. Therefore, the ability to thoroughly evaluate the angiogenic response following experimentally induced MI in rats, and distinguish it from inflammation, is desired. This would enable evaluation of the angiogenic potential of new therapeutics and improve knowledge on MI pathophysiology. Due to the complex response to MI involving multiple cell types and the limited selection of rat-specific antibodies, careful optimization is crucial to capture this complexity. Here, we present an 8-color flow cytometry-based multicolor panel that will enable quantification of the ongoing angiogenic response as well as characterize the cells involved. A detailed description of tissue preparation, immunostaining, and gating strategy is provided.

AB - Angiogenesis is involved in regeneration of cardiac tissue following acute myocardial infarction (MI), a disease often investigated in rat models. Therefore, the ability to thoroughly evaluate the angiogenic response following experimentally induced MI in rats, and distinguish it from inflammation, is desired. This would enable evaluation of the angiogenic potential of new therapeutics and improve knowledge on MI pathophysiology. Due to the complex response to MI involving multiple cell types and the limited selection of rat-specific antibodies, careful optimization is crucial to capture this complexity. Here, we present an 8-color flow cytometry-based multicolor panel that will enable quantification of the ongoing angiogenic response as well as characterize the cells involved. A detailed description of tissue preparation, immunostaining, and gating strategy is provided.

KW - angiogenesis

KW - flow cytometry

KW - immune response

KW - myocardial infarction

KW - rat cardiac tissue

KW - rat heart

U2 - 10.1002/cpz1.40

DO - 10.1002/cpz1.40

M3 - Journal article

C2 - 33570836

AN - SCOPUS:85102206402

VL - 1

JO - Current Protocols

JF - Current Protocols

SN - 2691-1299

IS - 2

M1 - e40

ER -

ID: 279627447