Estimation of the effective intercellular diffusion coefficient in cell monolayers coupled by gap junctions
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
A recently developed dye-based assay to study gap junction permeability is analysed. The assay is based on electroporation of dye into a large number of connexin 43 expressing cells, grown to confluency on electrically conductive slides. The subsequent intercellular spread of dye to non-electroporated parts of the monolayer enables estimation of the intercellular coupling. So far, the extent of dye spread has been analyzed in qualitative terms only and not in a manner based directly on the physics of the underlying diffusion process.
Originalsprog | Engelsk |
---|---|
Tidsskrift | European Journal of Pharmaceutical Sciences |
Vol/bind | 46 |
Udgave nummer | 4 |
Sider (fra-til) | 222-32 |
Antal sider | 11 |
ISSN | 0928-0987 |
DOI | |
Status | Udgivet - 2012 |
ID: 38229193