Effect of growth hormone and serum on the expression of the proto-oncogenes c-jun and c-fos in insulin producing cells

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Elisabeth D. Petersen, N Billestrup, Jens Høiriis Nielsen

Expression of the proto-oncogenes c-fos and c-jun was analysed in the insulin producing rat tumor cell line, RIN 5AH. Addition of fetal calf serum (FCS) to serum-starved cells in the presence of cycloheximid induced a modest increase in c-fos and c-jun mRNA levels, whereas growth hormone (GH) in the presence of cycloheximid had little or no effect, when added to RIN 5AH cells maintained in 0.5% FCS for 2 days prior to stimulation. Activation of protein kinase C by phorbol ester lead to increased c-jun and c-fos mRNA levels, whereas activation of adenylate cyclase by forskolin increased c-fos mRNA levels. These results suggest that the effects of GH on insulin producing cells are not mediated by activation of c-fos and c-jun transcription.
OriginalsprogEngelsk
TidsskriftBiomedica Biochimica Acta
Vol/bind49
Udgave nummer12
Sider (fra-til)1171-5
Antal sider5
ISSN0232-766X
StatusUdgivet - 1990

ID: 47974133