Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture

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Standard

Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. / Brunstedt, J; Nielsen, Jens Høiriis.

I: Acta endocrinologica, Bind 96, Nr. 4, 04.1981, s. 498-504.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Brunstedt, J & Nielsen, JH 1981, 'Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture', Acta endocrinologica, bind 96, nr. 4, s. 498-504.

APA

Brunstedt, J., & Nielsen, J. H. (1981). Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta endocrinologica, 96(4), 498-504.

Vancouver

Brunstedt J, Nielsen JH. Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. Acta endocrinologica. 1981 apr;96(4):498-504.

Author

Brunstedt, J ; Nielsen, Jens Høiriis. / Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. I: Acta endocrinologica. 1981 ; Bind 96, Nr. 4. s. 498-504.

Bibtex

@article{b48004c5a5b94061ba8e7c217dadfdd6,
title = "Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture",
abstract = "The effects of glucocorticoids on the pancreatic endocrine function was studied in isolated mouse pancreatic islets maintained in tissue culture for 1 to 3 weeks. Following culture for 2 week without corticoid supplement acute experiments with hydrocortisone showed no significant effect on the glucose-induced insulin release at 10(-8) to 10(-5) mol/l hydrocortisone. When, however, the islets were cultured in the presence of hydrocortisone, there was an increased insulin release to the medium in a dose-dependent manner, with the maximal effect at 10(-7) mol/l hydrocortisone. The release of glucagon to the medium was not affected to the same degree, but showed a slight inhibition at increasing concentrations of hydrocortisone. Short-term experiments after the culture period showed that islets cultured for 3 weeks in the presence of 10(-7) to 10(-5) mol/l hydrocortisone had an enhanced insulin secretion in response to glucose. The islets did not show any statistically significant change in their insulin- and DNA-content after 3 weeks of culture with hydrocortisone, but a marked reduction in the content of hydrocortisone. The present results suggest that physiological concentrations of hydrocortisone are importance for mouse islets to maintain their insulin production in tissue culture.",
keywords = "Animals, Culture Media, Culture Techniques, DNA, Glucagon, Hydrocortisone, Insulin, Islets of Langerhans, Male, Mice, Mice, Inbred Strains, Time Factors",
author = "J Brunstedt and Nielsen, {Jens H{\o}iriis}",
year = "1981",
month = apr,
language = "English",
volume = "96",
pages = "498--504",
journal = "European Journal of Endocrinology",
issn = "0804-4643",
publisher = "BioScientifica Ltd.",
number = "4",

}

RIS

TY - JOUR

T1 - Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture

AU - Brunstedt, J

AU - Nielsen, Jens Høiriis

PY - 1981/4

Y1 - 1981/4

N2 - The effects of glucocorticoids on the pancreatic endocrine function was studied in isolated mouse pancreatic islets maintained in tissue culture for 1 to 3 weeks. Following culture for 2 week without corticoid supplement acute experiments with hydrocortisone showed no significant effect on the glucose-induced insulin release at 10(-8) to 10(-5) mol/l hydrocortisone. When, however, the islets were cultured in the presence of hydrocortisone, there was an increased insulin release to the medium in a dose-dependent manner, with the maximal effect at 10(-7) mol/l hydrocortisone. The release of glucagon to the medium was not affected to the same degree, but showed a slight inhibition at increasing concentrations of hydrocortisone. Short-term experiments after the culture period showed that islets cultured for 3 weeks in the presence of 10(-7) to 10(-5) mol/l hydrocortisone had an enhanced insulin secretion in response to glucose. The islets did not show any statistically significant change in their insulin- and DNA-content after 3 weeks of culture with hydrocortisone, but a marked reduction in the content of hydrocortisone. The present results suggest that physiological concentrations of hydrocortisone are importance for mouse islets to maintain their insulin production in tissue culture.

AB - The effects of glucocorticoids on the pancreatic endocrine function was studied in isolated mouse pancreatic islets maintained in tissue culture for 1 to 3 weeks. Following culture for 2 week without corticoid supplement acute experiments with hydrocortisone showed no significant effect on the glucose-induced insulin release at 10(-8) to 10(-5) mol/l hydrocortisone. When, however, the islets were cultured in the presence of hydrocortisone, there was an increased insulin release to the medium in a dose-dependent manner, with the maximal effect at 10(-7) mol/l hydrocortisone. The release of glucagon to the medium was not affected to the same degree, but showed a slight inhibition at increasing concentrations of hydrocortisone. Short-term experiments after the culture period showed that islets cultured for 3 weeks in the presence of 10(-7) to 10(-5) mol/l hydrocortisone had an enhanced insulin secretion in response to glucose. The islets did not show any statistically significant change in their insulin- and DNA-content after 3 weeks of culture with hydrocortisone, but a marked reduction in the content of hydrocortisone. The present results suggest that physiological concentrations of hydrocortisone are importance for mouse islets to maintain their insulin production in tissue culture.

KW - Animals

KW - Culture Media

KW - Culture Techniques

KW - DNA

KW - Glucagon

KW - Hydrocortisone

KW - Insulin

KW - Islets of Langerhans

KW - Male

KW - Mice

KW - Mice, Inbred Strains

KW - Time Factors

M3 - Journal article

C2 - 7010864

VL - 96

SP - 498

EP - 504

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 4

ER -

ID: 47975442