Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture
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Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture. / Brunstedt, J; Nielsen, Jens Høiriis.
I: Acta Endocrinologica, Bind 96, Nr. 4, 04.1981, s. 498-504.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › fagfællebedømt
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TY - JOUR
T1 - Direct long-term effect of hydrocortisone on insulin and glucagon release from mouse pancreatic islets in tissue culture
AU - Brunstedt, J
AU - Nielsen, Jens Høiriis
PY - 1981/4
Y1 - 1981/4
N2 - The effects of glucocorticoids on the pancreatic endocrine function was studied in isolated mouse pancreatic islets maintained in tissue culture for 1 to 3 weeks. Following culture for 2 week without corticoid supplement acute experiments with hydrocortisone showed no significant effect on the glucose-induced insulin release at 10(-8) to 10(-5) mol/l hydrocortisone. When, however, the islets were cultured in the presence of hydrocortisone, there was an increased insulin release to the medium in a dose-dependent manner, with the maximal effect at 10(-7) mol/l hydrocortisone. The release of glucagon to the medium was not affected to the same degree, but showed a slight inhibition at increasing concentrations of hydrocortisone. Short-term experiments after the culture period showed that islets cultured for 3 weeks in the presence of 10(-7) to 10(-5) mol/l hydrocortisone had an enhanced insulin secretion in response to glucose. The islets did not show any statistically significant change in their insulin- and DNA-content after 3 weeks of culture with hydrocortisone, but a marked reduction in the content of hydrocortisone. The present results suggest that physiological concentrations of hydrocortisone are importance for mouse islets to maintain their insulin production in tissue culture.
AB - The effects of glucocorticoids on the pancreatic endocrine function was studied in isolated mouse pancreatic islets maintained in tissue culture for 1 to 3 weeks. Following culture for 2 week without corticoid supplement acute experiments with hydrocortisone showed no significant effect on the glucose-induced insulin release at 10(-8) to 10(-5) mol/l hydrocortisone. When, however, the islets were cultured in the presence of hydrocortisone, there was an increased insulin release to the medium in a dose-dependent manner, with the maximal effect at 10(-7) mol/l hydrocortisone. The release of glucagon to the medium was not affected to the same degree, but showed a slight inhibition at increasing concentrations of hydrocortisone. Short-term experiments after the culture period showed that islets cultured for 3 weeks in the presence of 10(-7) to 10(-5) mol/l hydrocortisone had an enhanced insulin secretion in response to glucose. The islets did not show any statistically significant change in their insulin- and DNA-content after 3 weeks of culture with hydrocortisone, but a marked reduction in the content of hydrocortisone. The present results suggest that physiological concentrations of hydrocortisone are importance for mouse islets to maintain their insulin production in tissue culture.
KW - Animals
KW - Culture Media
KW - Culture Techniques
KW - DNA
KW - Glucagon
KW - Hydrocortisone
KW - Insulin
KW - Islets of Langerhans
KW - Male
KW - Mice
KW - Mice, Inbred Strains
KW - Time Factors
M3 - Journal article
C2 - 7010864
VL - 96
SP - 498
EP - 504
JO - European Journal of Endocrinology
JF - European Journal of Endocrinology
SN - 0804-4643
IS - 4
ER -
ID: 47975442