Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects

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Standard

Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects. / Bruun, Jens M; Helge, Jørn W; Richelsen, Bjørn; Stallknecht, Bente.

I: American Journal of Physiology: Endocrinology and Metabolism, Bind 290, Nr. 5, 2006, s. E961-7.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Bruun, JM, Helge, JW, Richelsen, B & Stallknecht, B 2006, 'Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects', American Journal of Physiology: Endocrinology and Metabolism, bind 290, nr. 5, s. E961-7. https://doi.org/10.1152/ajpendo.00506.2005

APA

Bruun, J. M., Helge, J. W., Richelsen, B., & Stallknecht, B. (2006). Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects. American Journal of Physiology: Endocrinology and Metabolism, 290(5), E961-7. https://doi.org/10.1152/ajpendo.00506.2005

Vancouver

Bruun JM, Helge JW, Richelsen B, Stallknecht B. Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects. American Journal of Physiology: Endocrinology and Metabolism. 2006;290(5):E961-7. https://doi.org/10.1152/ajpendo.00506.2005

Author

Bruun, Jens M ; Helge, Jørn W ; Richelsen, Bjørn ; Stallknecht, Bente. / Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects. I: American Journal of Physiology: Endocrinology and Metabolism. 2006 ; Bind 290, Nr. 5. s. E961-7.

Bibtex

@article{e35245a0779011df928f000ea68e967b,
title = "Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects",
abstract = "Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet and daily exercise) on inflammatory markers in plasma, adipose tissue (AT), and skeletal muscle (SM) in 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, and vastus lateralis SM biopsies were obtained before and after the intervention and analyzed by ELISA and RT-PCR. The intervention reduced body weight (P < 0.001) and increased insulin sensitivity (homeostasis model assessment; P < 0.05). Plasma adiponectin (P < 0.001) increased, and C-reactive protein (P < 0.05), IL-6 (P < 0.01), IL-8 (P < 0.05), and monocyte chemoattractant protein-1 (P < 0.01) decreased. AT inflammation was reduced, determined from an increased mRNA expression of adiponectin (P < 0.001) and a decreased expression of macrophage-specific markers (CD14, CD68), IL-6, IL-8, and tumor necrosis factor-alpha (P < 0.01). After adjusting for macrophage infiltration in AT, only IL-6 mRNA was decreased (P < 0.05). Only very low levels of inflammatory markers were found in SM. The intervention had no effect on adiponectin receptor 1 and 2 mRNA in AT or SM. Thus hypocaloric diet and increased physical activity improved insulin sensitivity and reduced low-grade inflammation. Markers of inflammation were particularly reduced in AT, whereas SM does not contribute to this attenuation of whole body inflammation.",
author = "Bruun, {Jens M} and Helge, {J{\o}rn W} and Bj{\o}rn Richelsen and Bente Stallknecht",
note = "Keywords: Adiponectin; Adipose Tissue; Antigens, CD; Antigens, CD14; Antigens, Differentiation, Myelomonocytic; Blood Pressure; Body Weight; C-Reactive Protein; Chemokine CCL2; Diet; Exercise; Female; Gene Expression; Glucose Tolerance Test; Humans; Inflammation; Interleukin-6; Interleukin-8; Macrophages; Male; Muscle, Skeletal; Obesity; Receptors, Adiponectin; Receptors, Cell Surface; Tumor Necrosis Factor-alpha",
year = "2006",
doi = "10.1152/ajpendo.00506.2005",
language = "English",
volume = "290",
pages = "E961--7",
journal = "American Journal of Physiology: Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "5",

}

RIS

TY - JOUR

T1 - Diet and exercise reduce low-grade inflammation and macrophage infiltration in adipose tissue but not in skeletal muscle in severely obese subjects

AU - Bruun, Jens M

AU - Helge, Jørn W

AU - Richelsen, Bjørn

AU - Stallknecht, Bente

N1 - Keywords: Adiponectin; Adipose Tissue; Antigens, CD; Antigens, CD14; Antigens, Differentiation, Myelomonocytic; Blood Pressure; Body Weight; C-Reactive Protein; Chemokine CCL2; Diet; Exercise; Female; Gene Expression; Glucose Tolerance Test; Humans; Inflammation; Interleukin-6; Interleukin-8; Macrophages; Male; Muscle, Skeletal; Obesity; Receptors, Adiponectin; Receptors, Cell Surface; Tumor Necrosis Factor-alpha

PY - 2006

Y1 - 2006

N2 - Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet and daily exercise) on inflammatory markers in plasma, adipose tissue (AT), and skeletal muscle (SM) in 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, and vastus lateralis SM biopsies were obtained before and after the intervention and analyzed by ELISA and RT-PCR. The intervention reduced body weight (P < 0.001) and increased insulin sensitivity (homeostasis model assessment; P < 0.05). Plasma adiponectin (P < 0.001) increased, and C-reactive protein (P < 0.05), IL-6 (P < 0.01), IL-8 (P < 0.05), and monocyte chemoattractant protein-1 (P < 0.01) decreased. AT inflammation was reduced, determined from an increased mRNA expression of adiponectin (P < 0.001) and a decreased expression of macrophage-specific markers (CD14, CD68), IL-6, IL-8, and tumor necrosis factor-alpha (P < 0.01). After adjusting for macrophage infiltration in AT, only IL-6 mRNA was decreased (P < 0.05). Only very low levels of inflammatory markers were found in SM. The intervention had no effect on adiponectin receptor 1 and 2 mRNA in AT or SM. Thus hypocaloric diet and increased physical activity improved insulin sensitivity and reduced low-grade inflammation. Markers of inflammation were particularly reduced in AT, whereas SM does not contribute to this attenuation of whole body inflammation.

AB - Obesity is associated with low-grade inflammation, insulin resistance, type 2 diabetes, and cardiovascular disease. This study investigated the effect of a 15-wk lifestyle intervention (hypocaloric diet and daily exercise) on inflammatory markers in plasma, adipose tissue (AT), and skeletal muscle (SM) in 27 severely obese subjects (mean body mass index: 45.8 kg/m2). Plasma samples, subcutaneous abdominal AT biopsies, and vastus lateralis SM biopsies were obtained before and after the intervention and analyzed by ELISA and RT-PCR. The intervention reduced body weight (P < 0.001) and increased insulin sensitivity (homeostasis model assessment; P < 0.05). Plasma adiponectin (P < 0.001) increased, and C-reactive protein (P < 0.05), IL-6 (P < 0.01), IL-8 (P < 0.05), and monocyte chemoattractant protein-1 (P < 0.01) decreased. AT inflammation was reduced, determined from an increased mRNA expression of adiponectin (P < 0.001) and a decreased expression of macrophage-specific markers (CD14, CD68), IL-6, IL-8, and tumor necrosis factor-alpha (P < 0.01). After adjusting for macrophage infiltration in AT, only IL-6 mRNA was decreased (P < 0.05). Only very low levels of inflammatory markers were found in SM. The intervention had no effect on adiponectin receptor 1 and 2 mRNA in AT or SM. Thus hypocaloric diet and increased physical activity improved insulin sensitivity and reduced low-grade inflammation. Markers of inflammation were particularly reduced in AT, whereas SM does not contribute to this attenuation of whole body inflammation.

U2 - 10.1152/ajpendo.00506.2005

DO - 10.1152/ajpendo.00506.2005

M3 - Journal article

VL - 290

SP - E961-7

JO - American Journal of Physiology: Endocrinology and Metabolism

JF - American Journal of Physiology: Endocrinology and Metabolism

SN - 0193-1849

IS - 5

ER -

ID: 20293861