Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo

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Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo. / Herum, Kate Møller; Weng, Guangzheng; Kahnert, Konstantin; Waikel, Rebekah; Milburn, Greg; Conger, Autumn; Anaya, Paul; Campbell, Kenneth S.; Lundby, Alicia; Won, Kyoung Jae; Brakebusch, Cord.

I: Matrix Biology Plus, Bind 15, 100113, 2022.

Publikation: Bidrag til tidsskriftTidsskriftartikelfagfællebedømt

Harvard

Herum, KM, Weng, G, Kahnert, K, Waikel, R, Milburn, G, Conger, A, Anaya, P, Campbell, KS, Lundby, A, Won, KJ & Brakebusch, C 2022, 'Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo', Matrix Biology Plus, bind 15, 100113. https://doi.org/10.1016/j.mbplus.2022.100113

APA

Herum, K. M., Weng, G., Kahnert, K., Waikel, R., Milburn, G., Conger, A., Anaya, P., Campbell, K. S., Lundby, A., Won, K. J., & Brakebusch, C. (2022). Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo. Matrix Biology Plus, 15, [100113]. https://doi.org/10.1016/j.mbplus.2022.100113

Vancouver

Herum KM, Weng G, Kahnert K, Waikel R, Milburn G, Conger A o.a. Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo. Matrix Biology Plus. 2022;15. 100113. https://doi.org/10.1016/j.mbplus.2022.100113

Author

Herum, Kate Møller ; Weng, Guangzheng ; Kahnert, Konstantin ; Waikel, Rebekah ; Milburn, Greg ; Conger, Autumn ; Anaya, Paul ; Campbell, Kenneth S. ; Lundby, Alicia ; Won, Kyoung Jae ; Brakebusch, Cord. / Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo. I: Matrix Biology Plus. 2022 ; Bind 15.

Bibtex

@article{4bdd38620cc24982858d637c42cb348f,
title = "Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo",
abstract = "Many heart diseases are associated with fibrosis, but it is unclear whether different types of heart disease correlate with different subtypes of activated fibroblasts and to which extent such diversity is modeled during in vitro activation of primary cardiac fibroblasts. Analyzing the expression of 82 fibrosis related genes in 65 heart failure (HF) patients, we identified a panel of 12 genes clearly distinguishing HF patients better from healthy controls than measurement of the collagen-related hydroxyproline content. A subcluster enriched in ischemic HF was recognized, but not for diabetes, high BMI, or gender. Single-cell transcriptomic analysis of in vitro activated mouse cardiac fibroblasts distinguished 6 subpopulations, including a contractile Acta2high precursor population, which was predicted by time trajectory analysis to develop into Acta2low subpopulations with high production of extracellular matrix molecules. The 12 gene profile identified in HF patients showed highest similarity to the fibroblast subset with the strongest expression of extracellular matrix molecules. Population markers identified were furthermore able to clearly cluster different disease stages in a murine model for myocardial infarct. These data suggest that major features of cardiac fibroblast activation in heart failure patients, in murine heart disease models, and in cell culture of primary murine cardiac fibroblast are shared.",
keywords = "Fibrosis, Heart failure, Myofibroblast",
author = "Herum, {Kate M{\o}ller} and Guangzheng Weng and Konstantin Kahnert and Rebekah Waikel and Greg Milburn and Autumn Conger and Paul Anaya and Campbell, {Kenneth S.} and Alicia Lundby and Won, {Kyoung Jae} and Cord Brakebusch",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.mbplus.2022.100113",
language = "English",
volume = "15",
journal = "Matrix Biology Plus",
issn = "2590-0285",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Cardiac fibroblast sub-types in vitro reflect pathological cardiac remodeling in vivo

AU - Herum, Kate Møller

AU - Weng, Guangzheng

AU - Kahnert, Konstantin

AU - Waikel, Rebekah

AU - Milburn, Greg

AU - Conger, Autumn

AU - Anaya, Paul

AU - Campbell, Kenneth S.

AU - Lundby, Alicia

AU - Won, Kyoung Jae

AU - Brakebusch, Cord

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Many heart diseases are associated with fibrosis, but it is unclear whether different types of heart disease correlate with different subtypes of activated fibroblasts and to which extent such diversity is modeled during in vitro activation of primary cardiac fibroblasts. Analyzing the expression of 82 fibrosis related genes in 65 heart failure (HF) patients, we identified a panel of 12 genes clearly distinguishing HF patients better from healthy controls than measurement of the collagen-related hydroxyproline content. A subcluster enriched in ischemic HF was recognized, but not for diabetes, high BMI, or gender. Single-cell transcriptomic analysis of in vitro activated mouse cardiac fibroblasts distinguished 6 subpopulations, including a contractile Acta2high precursor population, which was predicted by time trajectory analysis to develop into Acta2low subpopulations with high production of extracellular matrix molecules. The 12 gene profile identified in HF patients showed highest similarity to the fibroblast subset with the strongest expression of extracellular matrix molecules. Population markers identified were furthermore able to clearly cluster different disease stages in a murine model for myocardial infarct. These data suggest that major features of cardiac fibroblast activation in heart failure patients, in murine heart disease models, and in cell culture of primary murine cardiac fibroblast are shared.

AB - Many heart diseases are associated with fibrosis, but it is unclear whether different types of heart disease correlate with different subtypes of activated fibroblasts and to which extent such diversity is modeled during in vitro activation of primary cardiac fibroblasts. Analyzing the expression of 82 fibrosis related genes in 65 heart failure (HF) patients, we identified a panel of 12 genes clearly distinguishing HF patients better from healthy controls than measurement of the collagen-related hydroxyproline content. A subcluster enriched in ischemic HF was recognized, but not for diabetes, high BMI, or gender. Single-cell transcriptomic analysis of in vitro activated mouse cardiac fibroblasts distinguished 6 subpopulations, including a contractile Acta2high precursor population, which was predicted by time trajectory analysis to develop into Acta2low subpopulations with high production of extracellular matrix molecules. The 12 gene profile identified in HF patients showed highest similarity to the fibroblast subset with the strongest expression of extracellular matrix molecules. Population markers identified were furthermore able to clearly cluster different disease stages in a murine model for myocardial infarct. These data suggest that major features of cardiac fibroblast activation in heart failure patients, in murine heart disease models, and in cell culture of primary murine cardiac fibroblast are shared.

KW - Fibrosis

KW - Heart failure

KW - Myofibroblast

U2 - 10.1016/j.mbplus.2022.100113

DO - 10.1016/j.mbplus.2022.100113

M3 - Journal article

C2 - 35719864

AN - SCOPUS:85131757289

VL - 15

JO - Matrix Biology Plus

JF - Matrix Biology Plus

SN - 2590-0285

M1 - 100113

ER -

ID: 313646400