Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication

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Standard

Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. / Mackey, Abigail L; Rasmussen, Lotte Klejs; Kadi, Fawzi; Schjerling, Peter; Helmark, Ida C; Ponsot, Elodie; Aagaard, Per; Durigan, João Luiz Q; Kjaer, Michael.

I: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Bind 30, Nr. 6, 02.03.2016, s. 2266-2281.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Harvard

Mackey, AL, Rasmussen, LK, Kadi, F, Schjerling, P, Helmark, IC, Ponsot, E, Aagaard, P, Durigan, JLQ & Kjaer, M 2016, 'Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication', FASEB journal : official publication of the Federation of American Societies for Experimental Biology, bind 30, nr. 6, s. 2266-2281. https://doi.org/10.1096/fj.201500198R

APA

Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., ... Kjaer, M. (2016). Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 30(6), 2266-2281. https://doi.org/10.1096/fj.201500198R

Vancouver

Mackey AL, Rasmussen LK, Kadi F, Schjerling P, Helmark IC, Ponsot E o.a. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2016 mar 2;30(6):2266-2281. https://doi.org/10.1096/fj.201500198R

Author

Mackey, Abigail L ; Rasmussen, Lotte Klejs ; Kadi, Fawzi ; Schjerling, Peter ; Helmark, Ida C ; Ponsot, Elodie ; Aagaard, Per ; Durigan, João Luiz Q ; Kjaer, Michael. / Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication. I: FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2016 ; Bind 30, Nr. 6. s. 2266-2281.

Bibtex

@article{1a8fd2247f2f4f4ab8c8953e4e2dfbd4,
title = "Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication",
abstract = "With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3{\%} vs. PLA, 19 ± 2{\%} (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.",
author = "Mackey, {Abigail L} and Rasmussen, {Lotte Klejs} and Fawzi Kadi and Peter Schjerling and Helmark, {Ida C} and Elodie Ponsot and Per Aagaard and Durigan, {Jo{\~a}o Luiz Q} and Michael Kjaer",
note = "{\circledC} FASEB.",
year = "2016",
month = "3",
day = "2",
doi = "10.1096/fj.201500198R",
language = "English",
volume = "30",
pages = "2266--2281",
journal = "F A S E B Journal",
issn = "0892-6638",
publisher = "Federation of American Societies for Experimental Biology",
number = "6",

}

RIS

TY - JOUR

T1 - Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication

AU - Mackey, Abigail L

AU - Rasmussen, Lotte Klejs

AU - Kadi, Fawzi

AU - Schjerling, Peter

AU - Helmark, Ida C

AU - Ponsot, Elodie

AU - Aagaard, Per

AU - Durigan, João Luiz Q

AU - Kjaer, Michael

N1 - © FASEB.

PY - 2016/3/2

Y1 - 2016/3/2

N2 - With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.

AB - With this study we investigated the role of nonsteroidal anti-inflammatory drugs (NSAIDs) in human skeletal muscle regeneration. Young men ingested NSAID [1200 mg/d ibuprofen (IBU)] or placebo (PLA) daily for 2 wk before and 4 wk after an electrical stimulation-induced injury to the leg extensor muscles of one leg. Muscle biopsies were collected from the vastus lateralis muscles before and after stimulation (2.5 h and 2, 7, and 30 d) and were assessed for satellite cells and regeneration by immunohistochemistry and real-time RT-PCR, and we also measured telomere length. After injury, and compared with PLA, IBU was found to augment the proportion of ActiveNotch1(+) satellite cells at 2 d [IBU, 29 ± 3% vs. PLA, 19 ± 2% (means ± sem)], satellite cell content at 7 d [IBU, 0.16 ± 0.01 vs. PLA, 0.12 ± 0.01 (Pax7(+) cells/fiber)], and to expedite muscle repair at 30 d. The PLA group displayed a greater proportion of embryonic myosin(+) fibers and a residual ∼2-fold increase in mRNA levels of matrix proteins (all P < 0.05). Endomysial collagen was also elevated with PLA at 30 d. Minimum telomere length shortening was not observed. In conclusion, ingestion of NSAID has a potentiating effect on Notch activation of satellite cells and muscle remodeling during large-scale regeneration of injured human skeletal muscle.-Mackey, A. L., Rasmussen, L. K., Kadi, F., Schjerling, P., Helmark, I. C., Ponsot, E., Aagaard, P., Durigan, J. L. Q., Kjaer, M. Activation of satellite cells and the regeneration of human skeletal muscle are expedited by ingestion of nonsteroidal anti-inflammatory medication.

U2 - 10.1096/fj.201500198R

DO - 10.1096/fj.201500198R

M3 - Journal article

C2 - 26936358

VL - 30

SP - 2266

EP - 2281

JO - F A S E B Journal

JF - F A S E B Journal

SN - 0892-6638

IS - 6

ER -

ID: 157201095