A Vasopressin-Induced Change in Prostaglandin Receptor Subtype Expression Explains the Differential Effect of PGE2 on AQP2 Expression

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  • Peter M.T. Deen
  • Michelle Boone
  • Horst Schweer
  • Olesen, Emma Tina Bisgaard
  • Claudia Carmone
  • Jack F.M. Wetzels
  • Robert A. Fenton
  • Marleen L.A. Kortenoeven

Arginine vasopressin (AVP) stimulates the concentration of renal urine by increasing the principal cell expression of aquaporin-2 (AQP2) water channels. Prostaglandin E2 (PGE2) and prostaglandin (PGF) increase the water absorption of the principal cell without AVP, but PGE2 decreases it in the presence of AVP. The underlying mechanism of this paradoxical response was investigated here. Mouse cortical collecting duct (mkpCCDc14) cells mimic principal cells as they endogenously express AQP2 in response to AVP. PGE2 increased AQP2 abundance without desmopressin (dDAVP), while in the presence of dDAVP, PGE2, and PGF reduced AQP2 abundance. dDAVP increased the cellular PGD2 and PGE2 release and decreased the PGF release. MpkCCD cells expressed mRNAs for the receptors of PGE2 (EP1/EP4), PGF2 (FP), and TxB2 (TP). Incubation with dDAVP increased the expression of EP1 and FP but decreased the expression of EP4. In the absence of dDAVP, incubation of mpkCCD cells with an EP4, but not EP1/3, agonist increased AQP2 abundance, and the PGE2-induced increase in AQP2 was blocked with an EP4 antagonist. Moreover, in the presence of dDAVP, an EP1/3, but not EP4, agonist decreased the AQP2 abundance, and the addition of EP1 antagonists prevented the PGE2-mediated downregulation of AQP2. Our study shows that in mpkCCDc14 cells, reduced EP4 receptor and increased EP1/FP receptor expression by dDAVP explains the differential effects of PGE2 and PGF on AQP2 abundance with or without dDAVP. As the V2R and EP4 receptor, but not the EP1 and FP receptor, can couple to Gs and stimulate the cyclic adenosine monophosphate (cAMP) pathway, our data support a view that cells can desensitize themselves for receptors activating the same pathway and sensitize themselves for receptors of alternative pathways.

TidsskriftFrontiers in Physiology
Antal sider13
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
PD is a recipient of VICI grant 865.07.0h02 of the Netherlands Organization for Scientific research (NWO). This work was supported by grants from NWO (VICI grant 865.07.002) and RUNMC (2004.55) to PD and grants from the Independent Research Fund Denmark (Project No. 1333-00279 and 1331-00738B) and the Aarhus University Research Foundation to MK. RF was funded by the Independent Research Fund Denmark (Project No. 1026-00063B) and the Novo Nordisk Foundation.

Funding Information:
We thank Johan van Burgsteden and Michiel van den Brand, Nijmegen, and Christian Westberg, Aarhus, for their excellent technical assistance.

Publisher Copyright:
Copyright © 2022 Deen, Boone, Schweer, Olesen, Carmone, Wetzels, Fenton and Kortenoeven.

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