The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse

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Standard

The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse. / Vasyutina, Elena; Martarelli, Benedetta; Brakebusch, Cord; Wende, Hagen; Birchmeier, Carmen.

I: Proceedings of the National Academy of Science of the United States of America, Bind 106, Nr. 22, 2009, s. 8935-40.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Vasyutina, E, Martarelli, B, Brakebusch, C, Wende, H & Birchmeier, C 2009, 'The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse', Proceedings of the National Academy of Science of the United States of America, bind 106, nr. 22, s. 8935-40. https://doi.org/10.1073/pnas.0902501106

APA

Vasyutina, E., Martarelli, B., Brakebusch, C., Wende, H., & Birchmeier, C. (2009). The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse. Proceedings of the National Academy of Science of the United States of America, 106(22), 8935-40. https://doi.org/10.1073/pnas.0902501106

Vancouver

Vasyutina E, Martarelli B, Brakebusch C, Wende H, Birchmeier C. The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse. Proceedings of the National Academy of Science of the United States of America. 2009;106(22):8935-40. https://doi.org/10.1073/pnas.0902501106

Author

Vasyutina, Elena ; Martarelli, Benedetta ; Brakebusch, Cord ; Wende, Hagen ; Birchmeier, Carmen. / The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse. I: Proceedings of the National Academy of Science of the United States of America. 2009 ; Bind 106, Nr. 22. s. 8935-40.

Bibtex

@article{07177f60625b11de8bc9000ea68e967b,
title = "The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse",
abstract = "Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42 mutant myoblasts correlates with a deficit in the recruitment of actin fibers and vinculin to myoblast contact sites. Comparison of the changes observed in mutant myogenic cells indicates that Rac1 and Cdc42 function in a nonredundant and not completely overlapping manner during the fusion process. Our genetic analysis demonstrates thus that the function of Rac in myoblast fusion is evolutionarily conserved from insects to mammals and that Cdc42, a molecule hitherto not implicated in myoblast fusion, is essential for the fusion of murine myoblasts.",
author = "Elena Vasyutina and Benedetta Martarelli and Cord Brakebusch and Hagen Wende and Carmen Birchmeier",
note = "Keywords: Animals; Cell Fusion; Drosophila; Evolution, Molecular; Mice; Mice, Transgenic; Mutagenesis; Myoblasts, Skeletal; cdc42 GTP-Binding Protein; rac1 GTP-Binding Protein",
year = "2009",
doi = "10.1073/pnas.0902501106",
language = "English",
volume = "106",
pages = "8935--40",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "22",

}

RIS

TY - JOUR

T1 - The small G-proteins Rac1 and Cdc42 are essential for myoblast fusion in the mouse

AU - Vasyutina, Elena

AU - Martarelli, Benedetta

AU - Brakebusch, Cord

AU - Wende, Hagen

AU - Birchmeier, Carmen

N1 - Keywords: Animals; Cell Fusion; Drosophila; Evolution, Molecular; Mice; Mice, Transgenic; Mutagenesis; Myoblasts, Skeletal; cdc42 GTP-Binding Protein; rac1 GTP-Binding Protein

PY - 2009

Y1 - 2009

N2 - Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42 mutant myoblasts correlates with a deficit in the recruitment of actin fibers and vinculin to myoblast contact sites. Comparison of the changes observed in mutant myogenic cells indicates that Rac1 and Cdc42 function in a nonredundant and not completely overlapping manner during the fusion process. Our genetic analysis demonstrates thus that the function of Rac in myoblast fusion is evolutionarily conserved from insects to mammals and that Cdc42, a molecule hitherto not implicated in myoblast fusion, is essential for the fusion of murine myoblasts.

AB - Rac1 and Cdc42 are small G-proteins that regulate actin dynamics and affect plasma membrane protrusion and vesicle traffic. We used conditional mutagenesis in mice to demonstrate that Rac1 and Cdc42 are essential for myoblast fusion in vivo and in vitro. The deficit in fusion of Rac1 or Cdc42 mutant myoblasts correlates with a deficit in the recruitment of actin fibers and vinculin to myoblast contact sites. Comparison of the changes observed in mutant myogenic cells indicates that Rac1 and Cdc42 function in a nonredundant and not completely overlapping manner during the fusion process. Our genetic analysis demonstrates thus that the function of Rac in myoblast fusion is evolutionarily conserved from insects to mammals and that Cdc42, a molecule hitherto not implicated in myoblast fusion, is essential for the fusion of murine myoblasts.

U2 - 10.1073/pnas.0902501106

DO - 10.1073/pnas.0902501106

M3 - Journal article

C2 - 19443691

VL - 106

SP - 8935

EP - 8940

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 22

ER -

ID: 12866257