Short-term exercise reduces markers of hepatocyte apoptosis in nonalcoholic fatty liver disease
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Short-term exercise reduces markers of hepatocyte apoptosis in nonalcoholic fatty liver disease. / Fealy, Ciaran E; Haus, Jacob M; Solomon, Thomas; Pagadala, Mangesh; Flask, Chris A; McCullough, Arthur J; Kirwan, John P.
I: Journal of Applied Physiology, Bind 113, Nr. 1, 07.2012, s. 1-6.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Short-term exercise reduces markers of hepatocyte apoptosis in nonalcoholic fatty liver disease
AU - Fealy, Ciaran E
AU - Haus, Jacob M
AU - Solomon, Thomas
AU - Pagadala, Mangesh
AU - Flask, Chris A
AU - McCullough, Arthur J
AU - Kirwan, John P
PY - 2012/7
Y1 - 2012/7
N2 - Increased hepatocyte apoptosis is a hallmark of nonalcoholic fatty liver disease (NAFLD) and contributes to the profibrogenic state responsible for the progression to nonalcoholic steatohepatitis (NASH). Strategies aimed at reducing apoptosis may result in better outcomes for individuals with NAFLD. We therefore examined the effect of a short-term exercise program on markers of apoptosis-plasma cytokeratin 18 (CK18) fragments, alanine aminotransferase (ALT), aspartate aminotransferase (AST), soluble Fas (sFas), and sFas ligand (sFasL)-in 13 obese individuals with NAFLD [body mass index 35.2 ± 1.2 kg/m(2), >5% intrahepatic lipid (IHL) assessed by (1)H-MR spectroscopy]. Exercise consisted of treadmill walking for 60 min/day on 7 consecutive days at ∼85% of maximal heart rate. Additionally, subjects underwent an oral glucose tolerance test and a maximal oxygen consumption (Vo(2max)) test before and after the exercise intervention. The Matsuda index was used to assess insulin sensitivity. We observed significant decreases in CK18 fragments (558.4 ± 106.8 vs. 323.4 ± 72.5 U/l, P <0.01) and ALT (30.2 ± 5.1 vs. 24.3 ± 4.8 U/l, P <0.05), and an increase in whole body fat oxidation (49.3 ± 6.1 vs. 69.4 ± 7.1 mg/min, P <0.05), while decreases in circulating sFasL approached statistical significance (66.5 ± 6.0 vs. 63.0 ± 5.7 pg/ml, P = 0.06), as did the relationship between percent change in circulating CK18 fragments and ALT (r = 0.55, P = 0.05). We also observed a significant correlation between changes in fat oxidation and circulating sFasL (rho = -0.65, P <0.05). There was no change in IHL following the intervention (18.2 ± 2.5 vs. 17.5 ± 2.1%, NS). We conclude that short-term exercise reduces a circulatory marker of hepatocyte apoptosis in obese individuals with NAFLD and propose that changes in the proapoptotic environment may be mediated through improved insulin sensitivity and increased oxidative capacity.
AB - Increased hepatocyte apoptosis is a hallmark of nonalcoholic fatty liver disease (NAFLD) and contributes to the profibrogenic state responsible for the progression to nonalcoholic steatohepatitis (NASH). Strategies aimed at reducing apoptosis may result in better outcomes for individuals with NAFLD. We therefore examined the effect of a short-term exercise program on markers of apoptosis-plasma cytokeratin 18 (CK18) fragments, alanine aminotransferase (ALT), aspartate aminotransferase (AST), soluble Fas (sFas), and sFas ligand (sFasL)-in 13 obese individuals with NAFLD [body mass index 35.2 ± 1.2 kg/m(2), >5% intrahepatic lipid (IHL) assessed by (1)H-MR spectroscopy]. Exercise consisted of treadmill walking for 60 min/day on 7 consecutive days at ∼85% of maximal heart rate. Additionally, subjects underwent an oral glucose tolerance test and a maximal oxygen consumption (Vo(2max)) test before and after the exercise intervention. The Matsuda index was used to assess insulin sensitivity. We observed significant decreases in CK18 fragments (558.4 ± 106.8 vs. 323.4 ± 72.5 U/l, P <0.01) and ALT (30.2 ± 5.1 vs. 24.3 ± 4.8 U/l, P <0.05), and an increase in whole body fat oxidation (49.3 ± 6.1 vs. 69.4 ± 7.1 mg/min, P <0.05), while decreases in circulating sFasL approached statistical significance (66.5 ± 6.0 vs. 63.0 ± 5.7 pg/ml, P = 0.06), as did the relationship between percent change in circulating CK18 fragments and ALT (r = 0.55, P = 0.05). We also observed a significant correlation between changes in fat oxidation and circulating sFasL (rho = -0.65, P <0.05). There was no change in IHL following the intervention (18.2 ± 2.5 vs. 17.5 ± 2.1%, NS). We conclude that short-term exercise reduces a circulatory marker of hepatocyte apoptosis in obese individuals with NAFLD and propose that changes in the proapoptotic environment may be mediated through improved insulin sensitivity and increased oxidative capacity.
KW - Alanine Transaminase
KW - Antigens, CD95
KW - Apoptosis
KW - Aspartate Aminotransferases
KW - Biological Markers
KW - Exercise Movement Techniques
KW - Fats
KW - Fatty Liver
KW - Female
KW - Glucose Tolerance Test
KW - Heart Rate
KW - Hepatocytes
KW - Humans
KW - Insulin Resistance
KW - Keratin-18
KW - Male
KW - Middle Aged
KW - Oxygen Consumption
KW - Walking
U2 - 10.1152/japplphysiol.00127.2012
DO - 10.1152/japplphysiol.00127.2012
M3 - Journal article
C2 - 22582214
VL - 113
SP - 1
EP - 6
JO - Journal of Applied Physiology
JF - Journal of Applied Physiology
SN - 8750-7587
IS - 1
ER -
ID: 50217967