Serological evaluation of Crimean-Congo hemorrhagic fever in humans with high-risk professions living in enzootic regions of Isfahan province of Iran and genetic analysis of circulating strains
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Serological evaluation of Crimean-Congo hemorrhagic fever in humans with high-risk professions living in enzootic regions of Isfahan province of Iran and genetic analysis of circulating strains. / Chinikar, Sadegh; Ghiasi, Seyed Mojtaba; Naddaf, Saeed; Piazak, Norair; Moradi, Maryam; Razavi, Mohammad R; Afzali, Neda; Haeri, Ali; Mostafavizadeh, Kamyar; Ataei, Behrouz; Khalilifard-Brojeni, Mohammad; Husseini, Sayed M; Bouloy, Michele.
I: Vector Borne and Zoonotic Diseases, Bind 12, Nr. 9, 09.2012, s. 733-8.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Serological evaluation of Crimean-Congo hemorrhagic fever in humans with high-risk professions living in enzootic regions of Isfahan province of Iran and genetic analysis of circulating strains
AU - Chinikar, Sadegh
AU - Ghiasi, Seyed Mojtaba
AU - Naddaf, Saeed
AU - Piazak, Norair
AU - Moradi, Maryam
AU - Razavi, Mohammad R
AU - Afzali, Neda
AU - Haeri, Ali
AU - Mostafavizadeh, Kamyar
AU - Ataei, Behrouz
AU - Khalilifard-Brojeni, Mohammad
AU - Husseini, Sayed M
AU - Bouloy, Michele
PY - 2012/9
Y1 - 2012/9
N2 - Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic viral disease that is asymptomatic in infected livestock, but causes a serious threat to humans with a mortality rate up to 50%. Although the CCHF virus (CCHFV) is often transmitted by ticks, livestock-to-human and human-to-human transmission also occurs. In the current study, we focused on CCHF in the province of Isfahan, located in the center of Iran and deemed to be the second most infected province. Human and livestock sera and resident ticks in the livestock are collected from different regions of the province and analyzed with specific IgG ELISA and RT-PCR tests. Overall, 12% and 12.7% of studied human and livestock populations were IgG positive, respectively. The genome of CCHFV was detected in 9% of ticks resident in livestock involved in this survey. The CCHFV isolates from infected ticks were genetically examined. Nucleotide sequence of the S-segment revealed that the different isolates were closely related to each other, with nucleotide sequence identities higher than 98%. Phylogenetic analysis demonstrated that a variant isolate clustered with the Iraq strain. This high proportion of IgG-positive sera and nearly high proportion of infected ticks increases the risk of CCHF outbreaks in the province and probably posits a great danger to other provinces.
AB - Crimean-Congo hemorrhagic fever (CCHF) is a zoonotic viral disease that is asymptomatic in infected livestock, but causes a serious threat to humans with a mortality rate up to 50%. Although the CCHF virus (CCHFV) is often transmitted by ticks, livestock-to-human and human-to-human transmission also occurs. In the current study, we focused on CCHF in the province of Isfahan, located in the center of Iran and deemed to be the second most infected province. Human and livestock sera and resident ticks in the livestock are collected from different regions of the province and analyzed with specific IgG ELISA and RT-PCR tests. Overall, 12% and 12.7% of studied human and livestock populations were IgG positive, respectively. The genome of CCHFV was detected in 9% of ticks resident in livestock involved in this survey. The CCHFV isolates from infected ticks were genetically examined. Nucleotide sequence of the S-segment revealed that the different isolates were closely related to each other, with nucleotide sequence identities higher than 98%. Phylogenetic analysis demonstrated that a variant isolate clustered with the Iraq strain. This high proportion of IgG-positive sera and nearly high proportion of infected ticks increases the risk of CCHF outbreaks in the province and probably posits a great danger to other provinces.
KW - Adult
KW - Aged
KW - Animals
KW - Antibodies, Viral
KW - Arachnid Vectors
KW - Cross-Sectional Studies
KW - Female
KW - Goat Diseases
KW - Goats
KW - Hemorrhagic Fever Virus, Crimean-Congo
KW - Hemorrhagic Fever, Crimean
KW - Humans
KW - Immunoglobulin G
KW - Iran
KW - Livestock
KW - Male
KW - Middle Aged
KW - Questionnaires
KW - RNA, Viral
KW - Sequence Analysis, DNA
KW - Seroepidemiologic Studies
KW - Sheep
KW - Sheep Diseases
KW - Ticks
KW - Young Adult
KW - Zoonoses
U2 - 10.1089/vbz.2011.0634
DO - 10.1089/vbz.2011.0634
M3 - Journal article
C2 - 22217167
VL - 12
SP - 733
EP - 738
JO - Vector Borne and Zoonotic Diseases
JF - Vector Borne and Zoonotic Diseases
SN - 1530-3667
IS - 9
ER -
ID: 132429920