Potentiation of insulin release in response to amino acid methyl esters correlates to activation of islet glutamate dehydrogenase activity
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Potentiation of insulin release in response to amino acid methyl esters correlates to activation of islet glutamate dehydrogenase activity. / Kofod, Hans; Lernmark, A; Hedeskov, C J.
I: Acta Physiologica Scandinavica. Supplementum, Bind 128, Nr. 3, 11.1986, s. 335-40.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Potentiation of insulin release in response to amino acid methyl esters correlates to activation of islet glutamate dehydrogenase activity
AU - Kofod, Hans
AU - Lernmark, A
AU - Hedeskov, C J
PY - 1986/11
Y1 - 1986/11
N2 - Column perifusion of mouse pancreatic islets was used to study the ability of amino acids and their methyl esters to influence insulin release and activate islet glutamate dehydrogenase activity. In the absence of L-glutamine, L-serine and the methyl ester of L-phenylalanine, but neither L-phenylalanine nor L-serine methyl ester, stimulate insulin secretion. In the presence of L-glutamine, however, the effect of L-serine was additive, while the methyl esters of L-serine and L-phenylalanine as well as native L-phenylalanine, potentiated the glucose-stimulated release of insulin. Measurements of islet glutamate dehydrogenase activity showed that only the two methyl esters of L-phenylalanine and L-serine activated the enzyme. It is concluded that the mechanism by which methyl esters of amino acids potentiate insulin release is most likely to be mediated by the activation of pancreatic beta-cell glutamate dehydrogenase activity.
AB - Column perifusion of mouse pancreatic islets was used to study the ability of amino acids and their methyl esters to influence insulin release and activate islet glutamate dehydrogenase activity. In the absence of L-glutamine, L-serine and the methyl ester of L-phenylalanine, but neither L-phenylalanine nor L-serine methyl ester, stimulate insulin secretion. In the presence of L-glutamine, however, the effect of L-serine was additive, while the methyl esters of L-serine and L-phenylalanine as well as native L-phenylalanine, potentiated the glucose-stimulated release of insulin. Measurements of islet glutamate dehydrogenase activity showed that only the two methyl esters of L-phenylalanine and L-serine activated the enzyme. It is concluded that the mechanism by which methyl esters of amino acids potentiate insulin release is most likely to be mediated by the activation of pancreatic beta-cell glutamate dehydrogenase activity.
KW - Amino Acids
KW - Animals
KW - Drug Synergism
KW - Esters
KW - Glucose
KW - Glutamate Dehydrogenase
KW - Insulin
KW - Islets of Langerhans
KW - Male
KW - Mice
KW - Mice, Inbred Strains
KW - Perfusion
KW - Structure-Activity Relationship
M3 - Journal article
C2 - 3538783
VL - 128
SP - 335
EP - 340
JO - Acta Physiologica Scandinavica
JF - Acta Physiologica Scandinavica
SN - 0001-6772
IS - 3
ER -
ID: 45575211