Pancreatic islet insulin secretion and metabolism in adult rats malnourished during neonatal life.
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Pancreatic islet insulin secretion and metabolism in adult rats malnourished during neonatal life. / Barbosa, Francisco B; Capito, Kirsten; Kofod, Hans; Thams, Peter.
I: British Journal of Nutrition, Bind 87, Nr. 2, 2002, s. 147-55.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Pancreatic islet insulin secretion and metabolism in adult rats malnourished during neonatal life.
AU - Barbosa, Francisco B
AU - Capito, Kirsten
AU - Kofod, Hans
AU - Thams, Peter
N1 - Keywords: Animals; Animals, Newborn; Body Weight; Culture Techniques; Glucose; Glycerolphosphate Dehydrogenase; Insulin; Islets of Langerhans; Male; Mitochondria; Organ Size; Oxidation-Reduction; Pancreas; Phosphatidylinositols; Phospholipases A; Phospholipases A2; Protein Deficiency; Rats; Rats, Wistar
PY - 2002
Y1 - 2002
N2 - Pancreatic islets were isolated from rats that had been nursed by dams fed with a control or an 8.7% protein diet during the first 12 d of the lactation period. Glucose-induced insulin secretion from islets in the 8.7% protein group was reduced 50%. The islet insulin and DNA content were similar, whereas the pancreatic insulin content was reduced by 30 % in the rats fed 8.7 % protein. In order to elucidate the mechanism responsible for the attenuation of insulin secretion, measurements were performed of the activity of several islet enzymes that had previously been supposed to be involved in the coupling of glucose stimulation to insulin secretion. Islet glucose oxidation was unaffected, but glucose-stimulated hydrolysis of phosphatidylinositol was reduced by one-third in the islets of rats fed 8.7% protein. The activity of mitochondrial glycerophosphate dehydrogenase was similar in islets of rats fed the 8.7% protein diet and those fed the control diet. The activity of Ca-independent phospholipase A2 was increased fourfold in the islets of rats fed 8.7% protein. It is concluded that impairment of glucose-induced insulin secretion in rats fed a low-protein diet may be caused by attenuation of islet phosphatidylinositol hydrolysis, and it is tentatively suggested that the increased activity of Ca-independent phospholipase A2 in islets of rats fed a low-protein diet may participate in the stimulation of apoptosis.
AB - Pancreatic islets were isolated from rats that had been nursed by dams fed with a control or an 8.7% protein diet during the first 12 d of the lactation period. Glucose-induced insulin secretion from islets in the 8.7% protein group was reduced 50%. The islet insulin and DNA content were similar, whereas the pancreatic insulin content was reduced by 30 % in the rats fed 8.7 % protein. In order to elucidate the mechanism responsible for the attenuation of insulin secretion, measurements were performed of the activity of several islet enzymes that had previously been supposed to be involved in the coupling of glucose stimulation to insulin secretion. Islet glucose oxidation was unaffected, but glucose-stimulated hydrolysis of phosphatidylinositol was reduced by one-third in the islets of rats fed 8.7% protein. The activity of mitochondrial glycerophosphate dehydrogenase was similar in islets of rats fed the 8.7% protein diet and those fed the control diet. The activity of Ca-independent phospholipase A2 was increased fourfold in the islets of rats fed 8.7% protein. It is concluded that impairment of glucose-induced insulin secretion in rats fed a low-protein diet may be caused by attenuation of islet phosphatidylinositol hydrolysis, and it is tentatively suggested that the increased activity of Ca-independent phospholipase A2 in islets of rats fed a low-protein diet may participate in the stimulation of apoptosis.
U2 - 10.1079/BJN2001489
DO - 10.1079/BJN2001489
M3 - Journal article
C2 - 11895167
VL - 87
SP - 147
EP - 155
JO - British Journal of Nutrition
JF - British Journal of Nutrition
SN - 0007-1145
IS - 2
ER -
ID: 8522117