Northern blotting analysis
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Northern blotting analysis. / Josefsen, Knud; Nielsen, Henrik.
Methods in Molecular Biology : RNA: Methods and Protocols. Bind 703 Springer Science+Business Media, 2011. s. 87-105.Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt
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TY - CHAP
T1 - Northern blotting analysis
AU - Josefsen, Knud
AU - Nielsen, Henrik
PY - 2011/1/1
Y1 - 2011/1/1
N2 - Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.
AB - Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound. Then, the membrane is analysed by hybridization to one or more specific probes that are labelled for subsequent detection. Northern blotting is relatively simple to perform, inexpensive, and not plagued by artefacts. Recent developments of hybridization membranes and buffers have resulted in increased sensitivity closing the gap to the more laborious nuclease protection experiments.
KW - Blotting, Northern
KW - Electrophoresis, Agar Gel
KW - Electrophoresis, Polyacrylamide Gel
KW - Formaldehyde
KW - Glyoxal
KW - Membranes, Artificial
KW - RNA
U2 - 10.1007/978-1-59745-248-9_7
DO - 10.1007/978-1-59745-248-9_7
M3 - Book chapter
C2 - 21125485
VL - 703
SP - 87
EP - 105
BT - Methods in Molecular Biology
PB - Springer Science+Business Media
ER -
ID: 33542407