No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts
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No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts. / Bayer, Monika L; Schjerling, Peter; Biskup, Edyta; Herchenhan, Andreas; Heinemeier, Katja M; Doessing, Simon; Krogsgaard, Michael; Kjær, Michael.
I: Mechanisms of Ageing and Development, Bind 133, Nr. 5, 05.2012, s. 246-54.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - No donor age effect of human serum on collagen synthesis signaling and cell proliferation of human tendon fibroblasts
AU - Bayer, Monika L
AU - Schjerling, Peter
AU - Biskup, Edyta
AU - Herchenhan, Andreas
AU - Heinemeier, Katja M
AU - Doessing, Simon
AU - Krogsgaard, Michael
AU - Kjær, Michael
N1 - Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
PY - 2012/5
Y1 - 2012/5
N2 - The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-ß1 (TGF-ß1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-ß1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells.
AB - The aging process of tendon tissue is associated with decreased collagen content and increased risk for injuries. An essential factor in tendon physiology is transforming growth factor-ß1 (TGF-ß1), which is presumed to be reduced systemically with advanced age. The aim of this study was to investigate whether human serum from elderly donors would have an inhibiting effect on the expression of collagen and collagen-related genes as well as on cell proliferative capacity in tendon cells from young individuals. There was no difference in systemic TGF-ß1 levels in serum obtained from young and elderly donors, and we found no difference in collagen expression when cells were subjected to human serum from elderly versus young donors. In addition, tendon cell proliferation was similar when culture medium was supplemented with serum of different donor age. These findings suggest that factors such as the cell intrinsic capacity or the tissue-specific environment rather than systemic circulating factors are important for functional capacity throughout life in human tendon cells.
KW - Adolescent
KW - Adult
KW - Aged
KW - Aging
KW - Cell Proliferation
KW - Cells, Cultured
KW - Collagen
KW - Fibroblasts
KW - Humans
KW - Male
KW - Middle Aged
KW - Serum
KW - Signal Transduction
KW - Tendons
KW - Transforming Growth Factor beta1
KW - Young Adult
U2 - 10.1016/j.mad.2012.02.002
DO - 10.1016/j.mad.2012.02.002
M3 - Journal article
C2 - 22395123
VL - 133
SP - 246
EP - 254
JO - Mechanisms of Ageing and Development
JF - Mechanisms of Ageing and Development
SN - 0047-6374
IS - 5
ER -
ID: 44914139