TY - JOUR

T1 - Mechanisms underlying epithelium-dependent relaxation in rat bronchioles

T2 - analogy to EDHF-type relaxation in rat pulmonary arteries

AU - Kroigaard, Christel

AU - Dalsgaard, Thomas

AU - Simonsen, Ulf

PY - 2010/4

Y1 - 2010/4

N2 - This study investigated the mechanisms underlying epithelium-derived hyperpolarizing factor (EpDHF)-type relaxation in rat bronchioles. Immunohistochemistry was performed, and rat bronchioles and pulmonary arteries were mounted in microvascular myographs for functional studies. An opener of small (SK(Ca)) and intermediate (IK(Ca))-conductance calcium-activated potassium channels, NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime) was used to induce EpDHF-type relaxation. IK(Ca) and SK(Ca)3 positive immunoreactions were observed mainly in the epithelium and endothelium of bronchioles and arteries, respectively. In 5-hydroxytryptamine (1 microM)-contracted bronchioles (828 +/- 20 microm, n = 84) and U46619 (0.03 microM)-contracted arteries (720 +/- 24 microm, n = 68), NS309 (0.001-10 microM) induced concentration-dependent relaxations that were reduced by epithelium/endothelium removal and by blocking IK(Ca) channels with charybdotoxin and in bronchioles also by blocking SK(Ca) channels with apamin. Inhibition of cyclooxygenase, nitric oxide synthase, and cytochrome 2C isoenzymes, or blockade of large (BK(Ca))-conductance calcium-activated potassium channels with iberiotoxin, failed to reduce NS309 relaxation. In contrast to the pulmonary arteries, relaxations to a beta(2)-adrenoceptor agonist, salbutamol, were reduced in bronchioles by removing the epithelium or blocking IK(Ca) and/or SK(Ca) channels. Extracellular K(+) (2-20 mM) induced relaxation in both bronchioles and arteries. An inhibitor of Na(+)-K(+)-ATPase, ouabain, abolished relaxations to NS309, salbutamol, and K(+). These results suggest that IK(Ca) and SK(Ca)3 channels are located in the epithelium of bronchioles and endothelium of pulmonary arteries. Analog to the endothelium-derived hyperpolarizing factor (EDHF)-type relaxation in pulmonary arteries, these channels may be involved in EpDHF-type relaxation of bronchioles caused by epithelial K(+) efflux followed by activation of Na(+)-K(+)-ATPase in the underlying smooth muscle layer.

AB - This study investigated the mechanisms underlying epithelium-derived hyperpolarizing factor (EpDHF)-type relaxation in rat bronchioles. Immunohistochemistry was performed, and rat bronchioles and pulmonary arteries were mounted in microvascular myographs for functional studies. An opener of small (SK(Ca)) and intermediate (IK(Ca))-conductance calcium-activated potassium channels, NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime) was used to induce EpDHF-type relaxation. IK(Ca) and SK(Ca)3 positive immunoreactions were observed mainly in the epithelium and endothelium of bronchioles and arteries, respectively. In 5-hydroxytryptamine (1 microM)-contracted bronchioles (828 +/- 20 microm, n = 84) and U46619 (0.03 microM)-contracted arteries (720 +/- 24 microm, n = 68), NS309 (0.001-10 microM) induced concentration-dependent relaxations that were reduced by epithelium/endothelium removal and by blocking IK(Ca) channels with charybdotoxin and in bronchioles also by blocking SK(Ca) channels with apamin. Inhibition of cyclooxygenase, nitric oxide synthase, and cytochrome 2C isoenzymes, or blockade of large (BK(Ca))-conductance calcium-activated potassium channels with iberiotoxin, failed to reduce NS309 relaxation. In contrast to the pulmonary arteries, relaxations to a beta(2)-adrenoceptor agonist, salbutamol, were reduced in bronchioles by removing the epithelium or blocking IK(Ca) and/or SK(Ca) channels. Extracellular K(+) (2-20 mM) induced relaxation in both bronchioles and arteries. An inhibitor of Na(+)-K(+)-ATPase, ouabain, abolished relaxations to NS309, salbutamol, and K(+). These results suggest that IK(Ca) and SK(Ca)3 channels are located in the epithelium of bronchioles and endothelium of pulmonary arteries. Analog to the endothelium-derived hyperpolarizing factor (EDHF)-type relaxation in pulmonary arteries, these channels may be involved in EpDHF-type relaxation of bronchioles caused by epithelial K(+) efflux followed by activation of Na(+)-K(+)-ATPase in the underlying smooth muscle layer.

KW - Albuterol

KW - Animals

KW - Aryl Hydrocarbon Hydroxylases

KW - Biological Factors

KW - Bronchioles

KW - Endothelium

KW - Enzyme Inhibitors

KW - Epithelium

KW - Fluorescent Antibody Technique

KW - In Vitro Techniques

KW - Indoles

KW - Ion Channel Gating

KW - Isoenzymes

KW - Male

KW - Nitric Oxide Synthase

KW - Oximes

KW - Potassium Channels, Calcium-Activated

KW - Protein Transport

KW - Pulmonary Artery

KW - Rats

KW - Rats, Wistar

KW - Sodium-Potassium-Exchanging ATPase

KW - Vasodilation

U2 - 10.1152/ajplung.00220.2009

DO - 10.1152/ajplung.00220.2009

M3 - Journal article

C2 - 20118301

VL - 298

SP - L531-42

JO - American Journal of Physiology - Lung Cellular and Molecular Physiology

JF - American Journal of Physiology - Lung Cellular and Molecular Physiology

SN - 1040-0605

IS - 4

ER -