Implications for the assay and biological activity of interleukin-4. Results of a WHO international collaborative study
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Five ampouled preparations of interleukin-4 (IL-4) have been evaluated by 36 laboratories in 14 countries for their suitability to serve as an international standard for this material in a joint international collaborative study for interleukin-3 (IL-3) and IL-4. The preparations were assayed in a wide range of in vitro bioassays and immunoassays. It is clear from the study that different recombinant preparations of IL-4 can have very different biological specific activities, including those from the same source (i.e., E. coli). In addition, immunoassay estimates of IL-4 levels did not correlate with those of bioassays, illustrating the fact that immunoassays do not necessarily measure active cytokine. It is of interest that the estimates provided by the different bioassays were less variable than those produced by the immunoassays, suggesting that bioassays can be as accurate, if not more so, than immunoassays. The large reduction in the variability of estimates with the inclusion of a single reference preparation clearly illustrates the need for a single standard to assay IL-4. On the basis of the results reported here, with the agreement of the participants of the study with the authorization of the Expert Committee on Biological Standardization (ECBS) on the World Health Organization (WHO) the preparation of IL-4 (88/656) was established as the international standard for interleukin-4 with an assigned unitage of 1000 IU/ampoule.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Immunological Methods |
| Vol/bind | 194 |
| Udgave nummer | 1 |
| Sider (fra-til) | 13-25 |
| Antal sider | 13 |
| ISSN | 0022-1759 |
| DOI | |
| Status | Udgivet - 17 jul. 1996 |
Bibliografisk note
Funding Information:
Dr. Robin Callard, Cellular Immunology Unit, Institute of Child Health, 30 Guilford Street, London WCIN lAH, UK Ms. K. Paik and Dr. John McKearn. Searle, 700 Chesterfield Village Parkway, St. Louis, MO 63193, USA Dr. Marie Rock and Ms. Kathleen Harper, Searle. 4901 Searle Parkway Room K-224, Skokie. IL 60077, USA Dr. Steven J. Swanson, Associate Principal Scientist, Schering-Plough Research Institute, 2015 Galloping Hill Road, Kenilworth, NJ 07033.0539, USA Dr. Jay P. Siegel, FDA, Woodmont II Building, Room 3047 HFM570. 1401 Rockville Pike. Rockville, MD 20852-1448, USA Dr. H.L. Spiegelberg, Immunology and Allergy Division, Departmento f Pediatrics 0609D. University of California, San Diego School of Medicine, San Diego, CA 92093-0609. USA Dr. John Geiger? and Kerri Carter. Immunex Manufacturing Corporation, 51 University Street, Seattle,W A 98101, USA Dr. William E. Paul and Cynthia Watson. National Institutes of Health, Building 10. Room 1l N3 11, 9000 Rockville Pike, Bethesda, MD 20892, USA Drs. Monica Tsang and Li Zhou, R & D Systems Inc., 614 McKinley Place NE, Minneapolis, MN 55413, USA Mr. Louis Westreich, Schering Plough Research Institute, 1011 Morris Avenue (U-2-A). Union, NJ 07083. USA Dr. Chun C. Chao, Minneapolis Medical Research Foundation. 914 South 8th Street, D3. Minneapolis, MN 55404, USA Drs. Terry L. Riss and Rich Moravec, Promega Corporation, 2800 Woods Hollow Road, Madison, WI 5371l -5399, USA
ID: 313376214