Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis. / Gupta, Shashank; Utoft, Regine Egeholm; Hasseldam, Henrik; Schmidt-Christensen, Anja; Hannibal, Tine Dahlbæk; Hansen, Lisbeth; Fransén Pettersson, Nina Isabell; Gupta, Noopur Agarwal; Rozell, Björn; Andersson, Åsa; Holmberg, Dan Ingemar.
I: PLOS ONE, Bind 8, Nr. 10, e76330, 10.2013, s. e76330.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Global and 3D Spatial Assessment of Neuroinflammation in Rodent Models of Multiple Sclerosis
AU - Gupta, Shashank
AU - Utoft, Regine Egeholm
AU - Hasseldam, Henrik
AU - Schmidt-Christensen, Anja
AU - Hannibal, Tine Dahlbæk
AU - Hansen, Lisbeth
AU - Fransén Pettersson, Nina Isabell
AU - Gupta, Noopur Agarwal
AU - Rozell, Björn
AU - Andersson, Åsa
AU - Holmberg, Dan Ingemar
PY - 2013/10
Y1 - 2013/10
N2 - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.
AB - Multiple Sclerosis (MS) is a progressive autoimmune inflammatory and demyelinating disease of the central nervous system (CNS). T cells play a key role in the progression of neuroinflammation in MS and also in the experimental autoimmune encephalomyelitis (EAE) animal models for the disease. A technology for quantitative and 3 dimensional (3D) spatial assessment of inflammation in this and other CNS inflammatory conditions is much needed. Here we present a procedure for 3D spatial assessment and global quantification of the development of neuroinflammation based on Optical Projection Tomography (OPT). Applying this approach to the analysis of rodent models of MS, we provide global quantitative data of the major inflammatory component as a function of the clinical course. Our data demonstrates a strong correlation between the development and progression of neuroinflammation and clinical disease in several mouse and a rat model of MS refining the information regarding the spatial dynamics of the inflammatory component in EAE. This method provides a powerful tool to investigate the effect of environmental and genetic forces and for assessing the therapeutic effects of drug therapy in animal models of MS and other neuroinflammatory/neurodegenerative disorders.
U2 - 10.1371/journal.pone.0076330
DO - 10.1371/journal.pone.0076330
M3 - Journal article
VL - 8
SP - e76330
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 10
M1 - e76330
ER -
ID: 51473043