Galanin and vasoactive intestinal polypeptide: coexistence and corelease from the vascularly perfused pig ileum during distension and chemical stimulation of the mucosa
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Galanin and vasoactive intestinal polypeptide : coexistence and corelease from the vascularly perfused pig ileum during distension and chemical stimulation of the mucosa. / Harling, H; Messell, T; Poulsen, Steen Seier; Holst, J J.
I: Digestion, Bind 50, Nr. 2, 1991, s. 61-71.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Galanin and vasoactive intestinal polypeptide
T2 - coexistence and corelease from the vascularly perfused pig ileum during distension and chemical stimulation of the mucosa
AU - Harling, H
AU - Messell, T
AU - Poulsen, Steen Seier
AU - Holst, J J
PY - 1991
Y1 - 1991
N2 - By immunohistochemistry and double staining technique, almost complete coexistence of galanin-like immunoreactivity (GAL-LI) and vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) was demonstrated in submucosal ganglionic cells and mucosal nerve fibers of the porcine ileum. The release of the two neuropeptides was studied in isolated, vascularly perfused pig ileum. Distension (increasing intraluminal pressure by 10 mm Hg) and intraluminal instillation of homologous gallbladder bile, amino acids, 0.1 M HCl, hypertonic NaCl (3,400 mosm x kg-1) and hypertonic glucose (1,100 mosm x kg-1) all increased the release of GAL-LI and VIP-LI into the venous effluent in parallel. Being the most potent stimulus, bile increased the output of GAL-LI from 0.74 +/- 0.12 to 2.44 +/- 0.81 pmol/min (mean +/- SE, p = 0.018) and the output of VIP-LI from 3.29 +/- 0.19 to 12.68 +/- 4.01 pmol/min (p less than 0.001), respectively. In conclusion, the coexistence and parallel release of GAL and VIP suggest that GAL/VIP neurons may be involved in intramural secretory and motor reflexes.
AB - By immunohistochemistry and double staining technique, almost complete coexistence of galanin-like immunoreactivity (GAL-LI) and vasoactive intestinal polypeptide-like immunoreactivity (VIP-LI) was demonstrated in submucosal ganglionic cells and mucosal nerve fibers of the porcine ileum. The release of the two neuropeptides was studied in isolated, vascularly perfused pig ileum. Distension (increasing intraluminal pressure by 10 mm Hg) and intraluminal instillation of homologous gallbladder bile, amino acids, 0.1 M HCl, hypertonic NaCl (3,400 mosm x kg-1) and hypertonic glucose (1,100 mosm x kg-1) all increased the release of GAL-LI and VIP-LI into the venous effluent in parallel. Being the most potent stimulus, bile increased the output of GAL-LI from 0.74 +/- 0.12 to 2.44 +/- 0.81 pmol/min (mean +/- SE, p = 0.018) and the output of VIP-LI from 3.29 +/- 0.19 to 12.68 +/- 4.01 pmol/min (p less than 0.001), respectively. In conclusion, the coexistence and parallel release of GAL and VIP suggest that GAL/VIP neurons may be involved in intramural secretory and motor reflexes.
KW - Animals
KW - Bile
KW - Galanin
KW - Ileum
KW - Immunoenzyme Techniques
KW - Intestinal Mucosa
KW - Neuropeptides
KW - Peptides
KW - Radioimmunoassay
KW - Reflex
KW - Swine
KW - Vasoactive Intestinal Peptide
M3 - Journal article
C2 - 1725160
VL - 50
SP - 61
EP - 71
JO - Digestion
JF - Digestion
SN - 0012-2823
IS - 2
ER -
ID: 47488053