Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils

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Standard

Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils. / Brasen, Jens Christian; Olsen, Lars Folke; Hallett, Maurice B.

I: B B A - Molecular Cell Research, Bind 1813, Nr. 8, 01.08.2011, s. 1446-52.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Brasen, JC, Olsen, LF & Hallett, MB 2011, 'Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils', B B A - Molecular Cell Research, bind 1813, nr. 8, s. 1446-52. https://doi.org/10.1016/j.bbamcr.2011.05.002

APA

Brasen, J. C., Olsen, L. F., & Hallett, M. B. (2011). Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils. B B A - Molecular Cell Research, 1813(8), 1446-52. https://doi.org/10.1016/j.bbamcr.2011.05.002

Vancouver

Brasen JC, Olsen LF, Hallett MB. Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils. B B A - Molecular Cell Research. 2011 aug. 1;1813(8):1446-52. https://doi.org/10.1016/j.bbamcr.2011.05.002

Author

Brasen, Jens Christian ; Olsen, Lars Folke ; Hallett, Maurice B. / Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils. I: B B A - Molecular Cell Research. 2011 ; Bind 1813, Nr. 8. s. 1446-52.

Bibtex

@article{06696e4250ab451a95abc1df6db2ae7f,

title = "Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils",

abstract = "In order to establish whether non-mitochondrial oxidase activity in human neutrophils is tightly related to cytosolic Ca(2+) concentration, we simultaneously measured Ca(2+) oscillations induced by ATP and oxidant production in single adherent neutrophils using confocal microscopy. ATP induced fast damped Ca(2+) spikes with a period of 15s and slower irregular spikes with a period greater than 50s. Spikes in Ca(2+) occurred in the absence of Ca(2+) influx, but the amplitude was damped by inhibition of Ca(2+) influx. Using the oxidation of hydroethidine as a cytosolic marker of oxidant production, we show that the generation of reactive oxygen species by neutrophils adherent to glass was accelerated by ATP. The step-up in NADPH oxidase activity followed the first elevation of cytosolic Ca(2+) but, despite subsequent spikes in Ca(2+) concentration, no oscillations in oxidase activity could be detected. ATP induced spikes in Ca(2+) in a very reproducible way and we propose that the Ca(2+) signal is an on-switch for oxidase activity, but the activity is apparently not directly correlated with spiking activity in cytosolic Ca(2+).",

author = "Brasen, {Jens Christian} and Olsen, {Lars Folke} and Hallett, {Maurice B}",

year = "2011",

month = aug,

day = "1",

doi = "10.1016/j.bbamcr.2011.05.002",

language = "English",

volume = "1813",

pages = "1446--52",

journal = "B B A - Molecular Cell Research",

issn = "0167-4889",

publisher = "Elsevier",

number = "8",

}

RIS

TY - JOUR

T1 - Extracellular ATP induces spikes in cytosolic free Ca(2+) but not in NADPH oxidase activity in neutrophils

AU - Brasen, Jens Christian

AU - Olsen, Lars Folke

AU - Hallett, Maurice B

PY - 2011/8/1

Y1 - 2011/8/1

N2 - In order to establish whether non-mitochondrial oxidase activity in human neutrophils is tightly related to cytosolic Ca(2+) concentration, we simultaneously measured Ca(2+) oscillations induced by ATP and oxidant production in single adherent neutrophils using confocal microscopy. ATP induced fast damped Ca(2+) spikes with a period of 15s and slower irregular spikes with a period greater than 50s. Spikes in Ca(2+) occurred in the absence of Ca(2+) influx, but the amplitude was damped by inhibition of Ca(2+) influx. Using the oxidation of hydroethidine as a cytosolic marker of oxidant production, we show that the generation of reactive oxygen species by neutrophils adherent to glass was accelerated by ATP. The step-up in NADPH oxidase activity followed the first elevation of cytosolic Ca(2+) but, despite subsequent spikes in Ca(2+) concentration, no oscillations in oxidase activity could be detected. ATP induced spikes in Ca(2+) in a very reproducible way and we propose that the Ca(2+) signal is an on-switch for oxidase activity, but the activity is apparently not directly correlated with spiking activity in cytosolic Ca(2+).

AB - In order to establish whether non-mitochondrial oxidase activity in human neutrophils is tightly related to cytosolic Ca(2+) concentration, we simultaneously measured Ca(2+) oscillations induced by ATP and oxidant production in single adherent neutrophils using confocal microscopy. ATP induced fast damped Ca(2+) spikes with a period of 15s and slower irregular spikes with a period greater than 50s. Spikes in Ca(2+) occurred in the absence of Ca(2+) influx, but the amplitude was damped by inhibition of Ca(2+) influx. Using the oxidation of hydroethidine as a cytosolic marker of oxidant production, we show that the generation of reactive oxygen species by neutrophils adherent to glass was accelerated by ATP. The step-up in NADPH oxidase activity followed the first elevation of cytosolic Ca(2+) but, despite subsequent spikes in Ca(2+) concentration, no oscillations in oxidase activity could be detected. ATP induced spikes in Ca(2+) in a very reproducible way and we propose that the Ca(2+) signal is an on-switch for oxidase activity, but the activity is apparently not directly correlated with spiking activity in cytosolic Ca(2+).

U2 - 10.1016/j.bbamcr.2011.05.002

DO - 10.1016/j.bbamcr.2011.05.002

M3 - Journal article

C2 - 21596069

VL - 1813

SP - 1446

EP - 1452

JO - B B A - Molecular Cell Research

JF - B B A - Molecular Cell Research

SN - 0167-4889

IS - 8

ER -

ID: 33684407

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