Expression of E-selectin, sialyl Lewis X, and macrophage inflammatory protein-1α by colonic epithelial cells in ulcerative colitis
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Expression of E-selectin, sialyl Lewis X, and macrophage inflammatory protein-1α by colonic epithelial cells in ulcerative colitis. / Vainer, Ben; Nielsen, Ole Haagen; Horn, Thomas.
I: Digestive Diseases and Sciences, Bind 43, Nr. 3, 18.04.1998, s. 596-608.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Expression of E-selectin, sialyl Lewis X, and macrophage inflammatory protein-1α by colonic epithelial cells in ulcerative colitis
AU - Vainer, Ben
AU - Nielsen, Ole Haagen
AU - Horn, Thomas
PY - 1998/4/18
Y1 - 1998/4/18
N2 - The pathogenic significance of cell adhesion molecules (CAMs) in ulcerative colitis (UC) is largely unknown. Colonic expression of E-selectin, sialyl Lewis X (sLe(x)), and macrophage inflammatory protein-1x (MIP-1α) as well as serum concentrations of E-selectin and MIP-1α in UC were studied. Thirty patients with UC, 10 patients with irritable bowel syndrome, and 10 healthy subjects were included. Colonic biopsies were stained immunohistochemically, and blood concentrations were measured with an ELISA technique. Soluble E-selectin did not correlate with diagnosis or disease activity, MIP-1α was below the detection limit. Epithelial cells expressed all three molecules, both on surface membranes and intracellularly. sLe(x) staining was weaker (P = 0.0002) and MIP-1α staining stronger (P = 0.014) in UC patients than in controls. Leukocyte MIP-α staining correlated with diagnosis (P = 0.021), sLe(x) staining (P = 0.023), and colonoscopy (P = 0.018). It is shown that E-selectin, sLe(x), and MIP-1α are synthesized and expressed by epithelial cells, indicating that CAMs are not only involved in leukocyte extravasation and migration, but also in the interaction between leukocytes and colonic epithelium. This knowledge might contribute to the development of improved treatments in UC.
AB - The pathogenic significance of cell adhesion molecules (CAMs) in ulcerative colitis (UC) is largely unknown. Colonic expression of E-selectin, sialyl Lewis X (sLe(x)), and macrophage inflammatory protein-1x (MIP-1α) as well as serum concentrations of E-selectin and MIP-1α in UC were studied. Thirty patients with UC, 10 patients with irritable bowel syndrome, and 10 healthy subjects were included. Colonic biopsies were stained immunohistochemically, and blood concentrations were measured with an ELISA technique. Soluble E-selectin did not correlate with diagnosis or disease activity, MIP-1α was below the detection limit. Epithelial cells expressed all three molecules, both on surface membranes and intracellularly. sLe(x) staining was weaker (P = 0.0002) and MIP-1α staining stronger (P = 0.014) in UC patients than in controls. Leukocyte MIP-α staining correlated with diagnosis (P = 0.021), sLe(x) staining (P = 0.023), and colonoscopy (P = 0.018). It is shown that E-selectin, sLe(x), and MIP-1α are synthesized and expressed by epithelial cells, indicating that CAMs are not only involved in leukocyte extravasation and migration, but also in the interaction between leukocytes and colonic epithelium. This knowledge might contribute to the development of improved treatments in UC.
KW - Cell adhesion molecules
KW - Colitis
KW - E-selectin
KW - Inflammatory bowel disease
KW - Macrophage inflammatory protein-1α
KW - Sialyl Lewis X
KW - Ulcerative
UR - http://www.scopus.com/inward/record.url?scp=15144341605&partnerID=8YFLogxK
U2 - 10.1023/A:1018875410987
DO - 10.1023/A:1018875410987
M3 - Journal article
C2 - 9539657
AN - SCOPUS:15144341605
VL - 43
SP - 596
EP - 608
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
SN - 0163-2116
IS - 3
ER -
ID: 218717800