Divalent metal transporter 1 knock-down modulates il-1β mediated pancreatic beta-cell pro-apoptotic signaling pathways through the autophagic machinery
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Divalent metal transporter 1 knock-down modulates il-1β mediated pancreatic beta-cell pro-apoptotic signaling pathways through the autophagic machinery. / Kang, Taewook; Huang, Honggang; Mandrup-Poulsen, Thomas; Larsen, Martin R.
I: International Journal of Molecular Sciences, Bind 22, Nr. 15, 8013, 2021.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Divalent metal transporter 1 knock-down modulates il-1β mediated pancreatic beta-cell pro-apoptotic signaling pathways through the autophagic machinery
AU - Kang, Taewook
AU - Huang, Honggang
AU - Mandrup-Poulsen, Thomas
AU - Larsen, Martin R.
N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021
Y1 - 2021
N2 - Pro-inflammatory cytokines promote cellular iron-import through enhanced divalent metal transporter-1 (DMT1) expression in pancreatic β-cells, consequently cell death. Inhibition of β-cell iron-import by DMT1 silencing protects against apoptosis in animal models of diabetes. However, how alterations of signaling networks contribute to the protective action of DMT1 knock-down is unknown. Here, we performed phosphoproteomics using our sequential enrichment strategy of mRNA, protein, and phosphopeptides, which enabled us to explore the concurrent molecular events in the same set of wildtype and DMT1-silenced β-cells during IL-1β exposure. Our findings reveal new phosphosites in the IL-1β-induced proteins that are clearly reverted by DMT1 silencing towards their steady-state levels. We validated the levels of five novel phosphosites of the potential protective proteins using parallel reaction monitoring. We also confirmed the inactivation of autophagic flux that may be relevant for cell survival induced by DMT1 silencing during IL-1β exposure. Additionally, the potential protective proteins induced by DMT1 silencing were related to insulin secretion that may lead to improving β-cell functions upon exposure to IL-1β. This global profiling has shed light on the signal transduction pathways driving the protection against inflammation-induced cell death in β-cells after DMT1 silencing.
AB - Pro-inflammatory cytokines promote cellular iron-import through enhanced divalent metal transporter-1 (DMT1) expression in pancreatic β-cells, consequently cell death. Inhibition of β-cell iron-import by DMT1 silencing protects against apoptosis in animal models of diabetes. However, how alterations of signaling networks contribute to the protective action of DMT1 knock-down is unknown. Here, we performed phosphoproteomics using our sequential enrichment strategy of mRNA, protein, and phosphopeptides, which enabled us to explore the concurrent molecular events in the same set of wildtype and DMT1-silenced β-cells during IL-1β exposure. Our findings reveal new phosphosites in the IL-1β-induced proteins that are clearly reverted by DMT1 silencing towards their steady-state levels. We validated the levels of five novel phosphosites of the potential protective proteins using parallel reaction monitoring. We also confirmed the inactivation of autophagic flux that may be relevant for cell survival induced by DMT1 silencing during IL-1β exposure. Additionally, the potential protective proteins induced by DMT1 silencing were related to insulin secretion that may lead to improving β-cell functions upon exposure to IL-1β. This global profiling has shed light on the signal transduction pathways driving the protection against inflammation-induced cell death in β-cells after DMT1 silencing.
KW - Anti-apoptosis
KW - Autophagy
KW - Cell cycle arrest
KW - Diabetes
KW - Iron metabolism
KW - ROS
UR - http://www.scopus.com/inward/record.url?scp=85111143589&partnerID=8YFLogxK
U2 - 10.3390/ijms22158013
DO - 10.3390/ijms22158013
M3 - Journal article
C2 - 34360779
AN - SCOPUS:85111143589
VL - 22
JO - International Journal of Molecular Sciences (CD-ROM)
JF - International Journal of Molecular Sciences (CD-ROM)
SN - 1424-6783
IS - 15
M1 - 8013
ER -
ID: 281102618