Digital image analysis: a review of reproducibility, stability and basic requirements for optimal results
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Digital image analysis : a review of reproducibility, stability and basic requirements for optimal results. / Riber-Hansen, Rikke; Vainer, Ben; Steiniche, Torben.
I: APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, Bind 120, Nr. 4, 04.2012, s. 276-89.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Digital image analysis
T2 - a review of reproducibility, stability and basic requirements for optimal results
AU - Riber-Hansen, Rikke
AU - Vainer, Ben
AU - Steiniche, Torben
N1 - © 2011 The Authors. APMIS © 2011 APMIS.
PY - 2012/4
Y1 - 2012/4
N2 - Digital image analysis (DIA) is increasingly implemented in histopathological research to facilitate truly quantitative measurements, decrease inter-observer variation and reduce hands-on time. Originally, efforts were made to enable DIA to reproduce manually obtained results on histological slides optimized for light microscopy and the human eye. With improved technical methods and the acknowledgement that computerized readings are different from analysis by human eye, recognition has been achieved that to really empower DIA, histological slides must be optimized for the digital 'eye', with reproducible results correlating with clinical findings. In this review, we focus on the basic expectations and requirements for DIA to gain wider use in histopathological research and diagnostics. With a reference to studies that specifically compare DIA with conventional methods, this review discusses reproducibility, application of stereology-based quantitative measurements, time consumption, optimization of histological slides, regions of interest selection and recent developments in staining and imaging techniques.
AB - Digital image analysis (DIA) is increasingly implemented in histopathological research to facilitate truly quantitative measurements, decrease inter-observer variation and reduce hands-on time. Originally, efforts were made to enable DIA to reproduce manually obtained results on histological slides optimized for light microscopy and the human eye. With improved technical methods and the acknowledgement that computerized readings are different from analysis by human eye, recognition has been achieved that to really empower DIA, histological slides must be optimized for the digital 'eye', with reproducible results correlating with clinical findings. In this review, we focus on the basic expectations and requirements for DIA to gain wider use in histopathological research and diagnostics. With a reference to studies that specifically compare DIA with conventional methods, this review discusses reproducibility, application of stereology-based quantitative measurements, time consumption, optimization of histological slides, regions of interest selection and recent developments in staining and imaging techniques.
KW - Biomedical Technology
KW - Diagnostic Imaging
KW - Humans
KW - Image Processing, Computer-Assisted
KW - Microscopy
KW - Reproducibility of Results
KW - Signal Processing, Computer-Assisted
KW - Staining and Labeling
U2 - 10.1111/j.1600-0463.2011.02854.x
DO - 10.1111/j.1600-0463.2011.02854.x
M3 - Journal article
C2 - 22429210
VL - 120
SP - 276
EP - 289
JO - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
JF - A P M I S. Acta Pathologica, Microbiologica et Immunologica Scandinavica
SN - 0903-4641
IS - 4
ER -
ID: 117547701